[Histonet] Help with Tunel

[Michael J. Lyon, Ph.D.]

I am looking for some help using the Chemicon ApopTag kit.  We have used
this successfully in the past on formalin fixed human muscle, frozen
sections.  We are looking at a different human muscle, processed similarly.
However our results are incredibly inconsistent even on the positive control
DNAse treated sections of the same muscle.  Some of the sections have
nothing at all.  While other sections have areas of very bright nuclei and
other areas are nearly negative.  

 

Any help appreciated.

 

Thanks

 

Michael J. Lyon, Ph.D.

Otolaryngology Research Lab

SUNY Upstate Medical University

750 East Adams Street

Syracuse, NY  13210

 

Voice 315-464-7253

Fax    315-464-5572