[Histonet] chattering
sheila adey
sheila_adey <@t> hotmail.com
Fri May 11 20:11:36 CDT 2007
Someone on histonet mentioned a 15 second soak on your water bath, then back
to the cold plate. It works wonders.
Sheila Adey HT MLT
Port Huron Hospital
Michigan
>From: LuAnn Anderson <ander093 <@t> tc.umn.edu>
>To: "Mike Pence" <mpence <@t> grhs.net>,"I-sanna Gibbons"
><trinimaican2501 <@t> yahoo.com>,<histonet <@t> lists.utsouthwestern.edu>
>Subject: RE: [Histonet] chattering
>Date: Fri, 11 May 2007 15:49:45 -0500
>
>I would soak the blocks, then make sure everything on the microtome is
>tightened down well (blade, block clamp etc.) and see if that will work.
>
>
>At 03:33 PM 5/11/2007, Mike Pence wrote:
>>Chatter can be from a number of reasons.
>>
>>Loose knife blade. Angle of knife not correct. Too "dry" of tissue.
>>(over fixed) Speed the tissue is cut across the blade. The material
>>the tissue is embedded in. The size of the tissue being cut. C.L.
>>Sturkey, Inc. Lebanon, PA. USA put out a paper about microtomy
>>trouble-shooting guide. See if you can locate that for reference.
>>
>>Mike
>>
>>-----Original Message-----
>>From: histonet-bounces <@t> lists.utsouthwestern.edu
>>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of I-sanna
>>Gibbons
>>Sent: Friday, May 11, 2007 3:16 PM
>>To: histonet <@t> lists.utsouthwestern.edu
>>Subject: [Histonet] chattering
>>
>>
>>Hi everyone, my problem is more chattering of the bat brain tissue
>>rather than cracking. Does chattering occur easily with the bat brain?
>>Waht are the causes of this chattering and how can I prevent it?
>> Thanks
>> I-sanna
>>
>> I-sanna Gibbons, DVM
>> Veterinary Anatomy
>> School of Veterinary Medicine
>> The University of the West Indies
>> Trinidad and Tobago, W.I.
>>
>>
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