[Histonet] (no subject)

[Anthony Gatt]

Hello, I am currently sectioning drosophila heads and am tearing up the paraffin.  Without the heads, I get beautiful ribbons so I suspect it is the sample itself.  I am using a tissue processor with the following protocol after an o/n fix in 4% PFA.

15m -alcoholic formalin (x2)
30m -95% EtOH (x2)
30m -100% EtOH (x2)
30m -Histoclear (x3)
30m -Paraffin (x2)
45m -Paraffin

Embed immediately in paraffin, cold block 30m, section next day.

I am new to this an would greatly appreciate any help.

Thank you,

Anthony