[Histonet] brain histology

[I-sanna Gibbons]

Thanks!  I'm always particularly concerned about safety.  The thing isI have tried other fixatives - 10% formal saline and buffered neutral formalin...  poor tissue integrity as well....

Sarah Jones <Sarah.Jones <@t> dako.com> wrote:  Why Carnoy's fixative? It contains chloroform, and therefore hardens
any tissues to the point of cracking. It is also very unsafe for you,
health-wise. I have been in this field for over 40 years, and worked
with chloroform for quite a bit of my early days in Histology. My liver
is greatly impaired today because of this. Of course, we had open
processors back then, and we also didn't have the OSHA regulations for
fume control that we have today. Personally, I would try a different
fixative.

Sarah A. Jones, HTL(ASCP)CM
Dako North America, Inc.
Carpinteria, CA. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of I-sanna
Gibbons
Sent: Wednesday, May 09, 2007 1:40 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] brain histology

I am having a bit of trouble obtaining good quality slides of bat
brains. The brains are fixed in Carnoy's, stored in absolute alcohol,
processed routinely, infiltrated with paraffin, sectioned at 8 microns
and stained using cresyl fast violet. The aim is to record the
cytoarchitecture. The resulting slides show poor tissue integrity with
extensive cracking. Can anyone suggest how this can be avoided? I was
thinking maybe a double-embedding method for whole brains (10-15 mm) and
thicker sections (25-30 microns)?

Thanking you in advance

I-sanna Gibbons, DVM
Veterinary Anatomy
School of Veterinary Medicine
The University of the West Indies
Trinidad and Tobago, W.I.


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