[Histonet] RE: timer calibration
Horn, Hazel V
HornHV <@t> archildrens.org
Thu May 10 13:55:22 CDT 2007
I think this is in the lab general checklist which applies to histology as well....
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall Slot 820
Little Rock, AR 72202
phone 501.364.4240
fax 501.364.3912
visit us on the web at: www.archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Shimp, Kristen R
Sent: Thursday, May 10, 2007 12:57 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: timer calibration
What CAP question number were you sited? I haven't heard of this one pertaining to histology timers.
Kristen
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, May 09, 2007 10:01 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 42, Issue 11
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Today's Topics:
1. Placentas (Cindy DuBois)
2. protein block (Till, Renee)
3. Re: protein block (Gayle Callis)
4. timer calibration (Margiotta, Michele)
5. If you wanted an antibody what would it be? (Patricia Adams)
6. Re: timer calibration (Rene J Buesa)
7. RE: [SPAM] [Histonet] reprocessing paraffin blocks
(Mickie Johnson)
8. RE: Regulatory Tcells (FoxP3) IHC (C.M. van der Loos)
9. RE: Leishmania (C.M. van der Loos)
10. FW: Her2 question (Cohen, Sherene B.)
11. Re: FW: Her2 question (Rene J Buesa)
12. Re: timer calibration (Joe Nocito)
13. RE: timer calibration (Mighnon Lashus)
14. RE: timer calibration (Douglas D Deltour)
15. Alizarin Red staining for mineralization nodules (docqian)
16. Leica ASP300 Tissue Processor for sale (Brian Branton)
17. RE: timer calibration (Mitchell Jean A.)
18. RE: timer calibration (Edwards, R.E.)
19. RE: protein block (Patsy Ruegg)
20. RE: timer calibration (Mike Pence)
21. (no subject) (Kennedy, Lisa)
22. Re: timer calibration (Bryan Hewlett)
23. RE: (no subject) (Mike Pence)
----------------------------------------------------------------------
Message: 1
Date: Tue, 8 May 2007 10:27:35 -0700 (PDT)
From: Cindy DuBois <dpahisto <@t> yahoo.com>
Subject: [Histonet] Placentas
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <769673.20813.qm <@t> web33409.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
We do this for one of our hospitals. We do charge the patient / insurance for a "Gross Only" on the placentas we accession as a gross only specimen.
We were told that since the child and / or parents can sue the hospital up to 20 years later for any perceived problems that may have arisen out of the pregnancy or labor, this was to cover the hospital legally.
The only hassle we have with this is how to store all the blocks we are producing.
Cindy DuBois
Stockton, CA
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Message: 2
Date: Tue, 8 May 2007 13:36:38 -0500
From: "Till, Renee" <TillRenee <@t> uams.edu>
Subject: [Histonet] protein block
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<11F927674DEBDC43B960809A7403C5D204550AA5 <@t> MAILPED.ad.uams.edu>
Content-Type: text/plain; charset=us-ascii
I have a protocol from a paper that I need to try and reproduce. They used
the Protein Block from Dako, along with the LSAB+ kit. The Block seems to no
longer be available. What would be an alternative? Is this the same as the
serum block I would normally use?
Renee' Till, HT
Research Assistant
Arkansas Children's Nutrition Center
1212 Marshall St./N2021
Little Rock, AR 72202
Lab (501)364-8504
Office Fax (501)364-3161
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Message: 3
Date: Tue, 08 May 2007 12:49:21 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] protein block
To: "Till, Renee" <TillRenee <@t> uams.edu>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20070508124600.01b06170 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Renee,
I doubt it, sometimes protein blocks are serum-free blocks, and may contain
BSA and/or casein. I would assume another company's block should do the
job as well. Try Lab Vision, Biocare, etc to see what they have available.
At 12:36 PM 5/8/2007, you wrote:
>I have a protocol from a paper that I need to try and reproduce. They used
>the Protein Block from Dako, along with the LSAB+ kit. The Block seems to no
>longer be available. What would be an alternative? Is this the same as the
>serum block I would normally use?
>
>
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
------------------------------
Message: 4
Date: Tue, 8 May 2007 15:42:12 -0400
From: "Margiotta, Michele" <MMargiotta <@t> bmhmc.org>
Subject: [Histonet] timer calibration
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
<922CE5B88F398948B4076A9A4340E7AF036AF5FF <@t> bmh_exchange.bmhmc.org>
Content-Type: text/plain; charset="iso-8859-1"
Hi All,
Does anyone have a procedure for calibrating timers? We just had an inspection and got cited because our timers were not calibrated. Any info would be appreciated!
Michele
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Message: 5
Date: Tue, 8 May 2007 13:11:02 -0700 (PDT)
From: Patricia Adams <alaskagirl1950 <@t> yahoo.com>
Subject: [Histonet] If you wanted an antibody what would it be?
To: HistoNet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <102360.41177.qm <@t> web52512.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
First I wish to thank everyone for helping me
with my species antibody problems.
My question is for all of you other Vet School
and Vet clinic people.
Do you have problems finding certain types of
antibodies that will react on certain species, if
so what antibody and which species?
I am trying to compile a list of the most needed
antibodies that we need on the animal side of
things.
I do again wish to thank all who sent me help, I
have been burning up the Internet looking up all
the information sent my way. And trying to dodge
my Pathologist and all his questions! (He knows
all my hiding places ;(, bummer!).
Patricia Adams
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Message: 6
Date: Tue, 8 May 2007 13:39:47 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] timer calibration
To: "Margiotta, Michele" <MMargiotta <@t> bmhmc.org>,
histonet <@t> pathology.swmed.edu
Message-ID: <113979.14134.qm <@t> web61212.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Try any local watch repair store. They have to calibrate their repaired watches so it is very likely they can help you.
René J.
"Margiotta, Michele" <MMargiotta <@t> bmhmc.org> wrote:
Hi All,
Does anyone have a procedure for calibrating timers? We just had an inspection and got cited because our timers were not calibrated. Any info would be appreciated!
Michele
This e-mail and any files transmitted with it are confidential and are intended
solely for the use of the individual or entity to which they are addressed.
This communication may contain material protected by the attorney-client
privilege. If you are not the intended recipient or the person responsible for
delivering the e-mail to the intended recipient, be advised that you have received
this e-mail in error and that any use, dissemination, forwarding, printing,
or copying of this e-mail is strictly prohibited. If you have received this e-mail in
error, please immediately notify the sender via return e-mail or call
Brookhaven Memorial Hospital Medical Center at (631) 654-7282.
_______________________________________________
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Message: 7
Date: Tue, 8 May 2007 18:32:59 -0700
From: "Mickie Johnson" <mickie25 <@t> netzero.net>
Subject: RE: [SPAM] [Histonet] reprocessing paraffin blocks
To: "'Gayle Callis'" <gcallis <@t> montana.edu>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<!&!AAAAAAAAAAAYAAAAAAAAAGSBPkiRJN1Ck0zqFBQmG77CgAAAEAAAACat7d15gRZEoNabyjQ9g5sBAAAAAA==@netzero.net>
Content-Type: text/plain; charset="us-ascii"
Hi Everyone. Thanks Gail for remembering my small article in Histo-Logic
about 5 years ago!
The procedure is very simple and the least harsh of any method I know. I did
not develop it, but one of my former students brought it back from NSH and
we used it with great success.
First, melt the block down and blot off the excess paraffin from the tissue.
Second, re-cassette the tissue in the same cassette, blotting excess
paraffin from the cassette.
Third, put the cassette in with the days normal tissue processing run, in
formalin.
Fourth, process as usual.
The next morning, embed and cut. The fat (or under processed tissue will cut
beautifully.
The rational is that the well processed part of the block will have paraffin
in it and will not feel the effects of dehydration. Xylene will melt out the
paraffin and then paraffin will re-infiltrate this part.
The unprocessed tissue areas are available to fix additionally and
dehydrate, clear and infiltrate with paraffin.
The net result is a reprocessed block with no harsh treatment and very
little time expended to 'reprocess' by hand. It does take overnight to get
the slides, but usually the pathologist will be happy to see slides he can
read accurately.
Hope this helps.
Good Luck!
Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
& Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
Web: www.mohshistotemp.com & www.mohslabstaffing.com
Email: mickie25 <@t> netzero.net
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle Callis
Sent: Friday, May 04, 2007 9:18 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [SPAM] [Histonet] reprocessing paraffin blocks
Get this publication from Histo Logic on Sakura Finetek website. It was
very simple and very little work overall.
<http://www.sakura-americas.com/histologic/articles/pdf/XXXVIno10503.pdf>A
Technique for Correcting Poorly Processed Paraffin Blocks.
Michael L. Johnson, BS, HTL, HT(ASCP), Spokane, WA, May 2003;XXXVI(1):21.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
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------------------------------
Message: 8
Date: Wed, 09 May 2007 09:19:03 +0200
From: "C.M. van der Loos" <c.m.vanderloos <@t> amc.uva.nl>
Subject: [Histonet] RE: Regulatory Tcells (FoxP3) IHC
To: histonet <@t> lists.utsouthwestern.edu
Cc: Melissa.Gonzalez <@t> cellgenesys.com, mauger <@t> email.chop.edu
Message-ID: <132f4512faf0.12faf0132f45 <@t> amc.uva.nl>
Content-Type: text/plain; charset="us-ascii"
Melissa,
We used the Abcam mouse antibody, clone 236A/E7 we great success on
both human cryo and FFPE samples, and also in double staining with
CD25, CD4 (cryo). For more details please see our paper recently
accepted for JHC: Onno J. de Boer et al., Immunohistochemical Analysis
of Regulatory T Cell Markers FOXP3 and GITR on CD4^+CD25^+ T Cells in
Normal Skin and Inflammatory Dermatoses. Go to the JHC website
([1]www.jhc.org) and go to exPRESS. You will find our paper under: May
3rd 2007.
Cheers,
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands
Date: Mon, 7 May 2007 10:35:52 -0700
From: "Melissa Gonzalez" <Melissa.Gonzalez <@t> cellgenesys.com>
Subject: [Histonet] Regulatory Tcells (FoxP3) IHC
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B9371E7140550D418212A1055BCD3E9E0C6EF4 <@t> hqsvr01mail.cgi.com>
Content-Type: text/plain; charset="us-ascii"
Hi all,
Anyone have any experience with this? How well does the Abcam antibody
work on FFPE human tissues? What's a good positive control?
Thanks
Melissa
References
1. http://www.jhc.org/
------------------------------
Message: 9
Date: Wed, 09 May 2007 09:50:37 +0200
From: "C.M. van der Loos" <c.m.vanderloos <@t> amc.uva.nl>
Subject: [Histonet] RE: Leishmania
To: histonet <@t> pathology.swmed.edu
Cc: SDrew <@t> uwhealth.org
Message-ID: <efebaec42c.ec42cefeba <@t> amc.uva.nl>
Content-Type: text/plain; charset="us-ascii"
Dear Sally,
Last year I tried an antibody from Cedarlane Leishmania LPG, clone
CA7AE (1:500, 60 min, RT). I could make it work with HIER citrate
pH6.0 and indirect fluorescence on FFPE samples. Spectral imaging was
used for unmixing "real" signal from autofluorescence. Even then it
was hard to find the specific signal, due to some specific-looking
nuclear background staining. The tissue samples that were positive
with fluorescence were also subjected to IHC with anti-mouse polymers
and DAB or LPR as chromogens. This totally failed and we gave up.
I realize it's not a very hopeful story but perhaps it helps anyway.
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands
Date: Mon, 7 May 2007 15:03:28 -0500
From: "Drew Sally A." <SDrew <@t> uwhealth.org>
Subject: [Histonet] Leishmania
To: "Histonet" <histonet <@t> pathology.swmed.edu>
Could someone direct us to a place/person who might run an antibody
against Leishmania? We
have a pathologist asking questions about it, and all our usual source
don't list it.
Thank you!
Sally Ann Drew, MT(ASCP)
IHC/ISH Laboratory
University of Wisconsin Hosp. & Clinics
Madison, WI 53792
------------------------------
Message: 10
Date: Wed, 9 May 2007 09:30:05 -0400
From: "Cohen, Sherene B." <Sherene.Cohen <@t> fccc.edu>
Subject: [Histonet] FW: Her2 question
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<670B8345AF238F40910FEC4CA7D4B7D2F9E991 <@t> exchserver.fccc.edu>
Content-Type: text/plain; charset="iso-8859-1"
> -----Original Message-----
> From: Cohen, Sherene B.
> Sent: Tuesday, May 08, 2007 3:12 PM
> To: 'histonet <@t> lists.utsouthwestern.edu'
> Subject: Her2 question
>
> Hey netters,
>
> My director just asked me about fixation in NBF and Her2. I'm curious to
> know what others are doing about:
>
> A) Weekend processing of breast specimens
>
> B) If anyone has studied the antigenicity effect of these specimens
> sitting in warm paraffin for at least 8 hours.
>
> Any feedeback is greatly appreciated.
>
> Sherene
>
>
------------------------------
Message: 11
Date: Wed, 9 May 2007 07:35:01 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] FW: Her2 question
To: "Cohen, Sherene B." <Sherene.Cohen <@t> fccc.edu>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <222753.88085.qm <@t> web61222.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
This was the subject of an extense discussion in Histonet just one month ago.
It would be better for you to look in the archieves and benefit from that discussion.
René J.
"Cohen, Sherene B." <Sherene.Cohen <@t> fccc.edu> wrote:
> -----Original Message-----
> From: Cohen, Sherene B.
> Sent: Tuesday, May 08, 2007 3:12 PM
> To: 'histonet <@t> lists.utsouthwestern.edu'
> Subject: Her2 question
>
> Hey netters,
>
> My director just asked me about fixation in NBF and Her2. I'm curious to
> know what others are doing about:
>
> A) Weekend processing of breast specimens
>
> B) If anyone has studied the antigenicity effect of these specimens
> sitting in warm paraffin for at least 8 hours.
>
> Any feedeback is greatly appreciated.
>
> Sherene
>
>
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 12
Date: Mon, 14 May 2007 10:08:30 -0500
From: "Joe Nocito" <jnocito <@t> satx.rr.com>
Subject: Re: [Histonet] timer calibration
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>, "Margiotta, Michele"
<MMargiotta <@t> bmhmc.org>, <histonet <@t> pathology.swmed.edu>
Message-ID: <00c801c79639$bc0a91a0$d49eae18 <@t> yourxhtr8hvc4p>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
I'm sorry
am I the only one that thinks calibrating timers is stupid. I mean how many
histology procedures are so time sensitive that the timers have to be
calibrated? Let's face it. I have had techs sit there and watch the clock
and rinse as soon as the timer goes off. And I've had techs wait for the
timer to go off , then mosey over and rinse. Both stains worked. I guess
I'm just not anal enough.
JTT
----- Original Message -----
From: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
To: "Margiotta, Michele" <MMargiotta <@t> bmhmc.org>;
<histonet <@t> pathology.swmed.edu>
Sent: Tuesday, May 08, 2007 3:39 PM
Subject: Re: [Histonet] timer calibration
> Try any local watch repair store. They have to calibrate their repaired
> watches so it is very likely they can help you.
> René J.
>
> "Margiotta, Michele" <MMargiotta <@t> bmhmc.org> wrote:
> Hi All,
>
> Does anyone have a procedure for calibrating timers? We just had an
> inspection and got cited because our timers were not calibrated. Any info
> would be appreciated!
>
> Michele
>
>
>
> This e-mail and any files transmitted with it are confidential and are
> intended
> solely for the use of the individual or entity to which they are
> addressed.
> This communication may contain material protected by the attorney-client
> privilege. If you are not the intended recipient or the person responsible
> for
> delivering the e-mail to the intended recipient, be advised that you have
> received
> this e-mail in error and that any use, dissemination, forwarding,
> printing,
> or copying of this e-mail is strictly prohibited. If you have received
> this e-mail in
> error, please immediately notify the sender via return e-mail or call
> Brookhaven Memorial Hospital Medical Center at (631) 654-7282.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Ahhh...imagining that irresistible "new car" smell?
> Check outnew cars at Yahoo! Autos.
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Wed, 9 May 2007 10:18:51 -0500
From: "Mighnon Lashus" <MLashus <@t> pathgroup.com>
Subject: RE: [Histonet] timer calibration
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<7DFAF4868AAAC34C986DF7E1AC16D02601156D54 <@t> pgnexchg1.pathgroup.com>
Content-Type: text/plain; charset="iso-8859-1"
Who sited you?
Mighnon
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joe Nocito
Sent: Monday, May 14, 2007 11:09 AM
To: Rene J Buesa; Margiotta, Michele; histonet <@t> pathology.swmed.edu
Subject: Re: [Histonet] timer calibration
I'm sorry
am I the only one that thinks calibrating timers is stupid. I mean how many
histology procedures are so time sensitive that the timers have to be
calibrated? Let's face it. I have had techs sit there and watch the clock
and rinse as soon as the timer goes off. And I've had techs wait for the
timer to go off , then mosey over and rinse. Both stains worked. I guess
I'm just not anal enough.
JTT
----- Original Message -----
From: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
To: "Margiotta, Michele" <MMargiotta <@t> bmhmc.org>;
<histonet <@t> pathology.swmed.edu>
Sent: Tuesday, May 08, 2007 3:39 PM
Subject: Re: [Histonet] timer calibration
> Try any local watch repair store. They have to calibrate their repaired
> watches so it is very likely they can help you.
> René J.
>
> "Margiotta, Michele" <MMargiotta <@t> bmhmc.org> wrote:
> Hi All,
>
> Does anyone have a procedure for calibrating timers? We just had an
> inspection and got cited because our timers were not calibrated. Any info
> would be appreciated!
>
> Michele
>
>
>
> This e-mail and any files transmitted with it are confidential and are
> intended
> solely for the use of the individual or entity to which they are
> addressed.
> This communication may contain material protected by the attorney-client
> privilege. If you are not the intended recipient or the person responsible
> for
> delivering the e-mail to the intended recipient, be advised that you have
> received
> this e-mail in error and that any use, dissemination, forwarding,
> printing,
> or copying of this e-mail is strictly prohibited. If you have received
> this e-mail in
> error, please immediately notify the sender via return e-mail or call
> Brookhaven Memorial Hospital Medical Center at (631) 654-7282.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Ahhh...imagining that irresistible "new car" smell?
> Check outnew cars at Yahoo! Autos.
> _______________________________________________
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Message: 14
Date: Wed, 9 May 2007 11:25:31 -0500
From: "Douglas D Deltour" <doug <@t> ppspath.com>
Subject: RE: [Histonet] timer calibration
To: "'Joe Nocito'" <jnocito <@t> satx.rr.com>,
<histonet <@t> pathology.swmed.edu>
Message-ID: <1033394974-763687002 <@t> pathology.swmed.edu>
Content-Type: text/plain; charset="iso-8859-1"
Who inspects the calibrating timers?
What are the calibrating timers calibrated against? What are the calibrating
timers of the calibrating timers calibrated against?
Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
(803)252-1913
Fax (803)254-3262
*****************************************************
PROFESSIONAL PATHOLOGY SERVICES, PC
NOTICE OF CONFIDENTIALITY
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law. If the reader
of this message is not the intended recipient, you are hereby notified that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law. If you have received this communication in
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joe Nocito
Sent: Monday, May 14, 2007 10:09 AM
To: Rene J Buesa; Margiotta, Michele; histonet <@t> pathology.swmed.edu
Subject: Re: [Histonet] timer calibration
I'm sorry
am I the only one that thinks calibrating timers is stupid. I mean how many
histology procedures are so time sensitive that the timers have to be
calibrated? Let's face it. I have had techs sit there and watch the clock
and rinse as soon as the timer goes off. And I've had techs wait for the
timer to go off , then mosey over and rinse. Both stains worked. I guess
I'm just not anal enough.
JTT
----- Original Message -----
From: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
To: "Margiotta, Michele" <MMargiotta <@t> bmhmc.org>;
<histonet <@t> pathology.swmed.edu>
Sent: Tuesday, May 08, 2007 3:39 PM
Subject: Re: [Histonet] timer calibration
> Try any local watch repair store. They have to calibrate their repaired
> watches so it is very likely they can help you.
> René J.
>
> "Margiotta, Michele" <MMargiotta <@t> bmhmc.org> wrote:
> Hi All,
>
> Does anyone have a procedure for calibrating timers? We just had an
> inspection and got cited because our timers were not calibrated. Any info
> would be appreciated!
>
> Michele
>
>
>
> This e-mail and any files transmitted with it are confidential and are
> intended
> solely for the use of the individual or entity to which they are
> addressed.
> This communication may contain material protected by the attorney-client
> privilege. If you are not the intended recipient or the person responsible
> for
> delivering the e-mail to the intended recipient, be advised that you have
> received
> this e-mail in error and that any use, dissemination, forwarding,
> printing,
> or copying of this e-mail is strictly prohibited. If you have received
> this e-mail in
> error, please immediately notify the sender via return e-mail or call
> Brookhaven Memorial Hospital Medical Center at (631) 654-7282.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Ahhh...imagining that irresistible "new car" smell?
> Check outnew cars at Yahoo! Autos.
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------------------------------
Message: 15
Date: Wed, 9 May 2007 23:24:51 +0800 (CST)
From: docqian <pex0220 <@t> yahoo.com.cn>
Subject: [Histonet] Alizarin Red staining for mineralization nodules
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <628956.75919.qm <@t> web15210.mail.cnb.yahoo.com>
Content-Type: text/plain; charset=gb2312
Dear all,
I would like to perform Alizarin Red staining for mineralization nodules in primary osteoblasts, Does anyone have a protocol about it?
In addition, for mineralization nodule formation, we should add ascorbic acid and beta-glycerolphosphate into the culture medium, my question is : use which solution to dissovle these drugs (distilled water, or culture medium).
Thank you.
Guofeng
___________________________________________________________
ÇÀ×¢ÑÅ»¢Ãâ·ÑÓÊÏä3.5GÈÝÁ¿£¬20M¸½¼þ£¡
http://cn.mail.yahoo.com
------------------------------
Message: 16
Date: Wed, 9 May 2007 11:30:39 -0400
From: "Brian Branton" <BBranton <@t> sarapath.com>
Subject: [Histonet] Leica ASP300 Tissue Processor for sale
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<2A5183C7E289F646A4744C85841587BF04128A <@t> timeclock.sarapath.com>
Content-Type: text/plain; charset="iso-8859-1"
Hello Histonet,
We are selling one of our Leica ASP300 tissue processors. If anyone is interested, please checkout our eBay listing http://cgi.ebay.com/Leica-ASP300-Tissue-Processor_W0QQitemZ200106385547QQihZ010QQcategoryZ11816QQssPageNameZWDVWQQrdZ1QQcmdZViewItem.
Thank You
Brian Branton
Purchasing Agent
Sarasota Pathology
(941) 362-8963
(941) 362-8964 FAX
------------------------------
Message: 17
Date: Wed, 9 May 2007 10:45:00 -0500
From: "Mitchell Jean A." <JMitchell <@t> uwhealth.org>
Subject: RE: [Histonet] timer calibration
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
<936BDBD9AB6ED84FB1FD25FD55DCDFB1035585A2 <@t> uwhis-xchng4.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
JCAHO inspectors each and every time have inquired about my timer
calibrations. I satisfy this requirement by annually calibrating my
timers to my wall clock; with the wall clocks calibrated to Greenwich
Mean Time (from a website)
Jean Mitchell, BS HT (ASCP)
University of Wisconsin Hospital & Clinics
Neuromuscular Laboratory Manager
600 Highland Avenue
Madison, WI 53792-5132
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Douglas
D Deltour
Sent: Wednesday, May 09, 2007 11:26 AM
To: 'Joe Nocito'; histonet <@t> pathology.swmed.edu
Subject: RE: [Histonet] timer calibration
Who inspects the calibrating timers?
What are the calibrating timers calibrated against? What are the
calibrating timers of the calibrating timers calibrated against?
Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
(803)252-1913
Fax (803)254-3262
------------------------------
Message: 18
Date: Wed, 9 May 2007 16:58:38 +0100
From: "Edwards, R.E." <ree3 <@t> leicester.ac.uk>
Subject: RE: [Histonet] timer calibration
To: "Joe Nocito" <jnocito <@t> satx.rr.com>, "Rene J Buesa"
<rjbuesa <@t> yahoo.com>, "Margiotta, Michele" <MMargiotta <@t> bmhmc.org>,
<histonet <@t> pathology.swmed.edu>
Message-ID:
<DC88BEDFD1FC3F468D0376A7C75465F70BD8FF11 <@t> Saffron.cfs.le.ac.uk>
Content-Type: text/plain; charset="iso-8859-1"
Calibration of timers is essential, as one of the least publicised consequences of global warming is that time is speeding up by about a second a year( Wells et al;Journal of Man and Time Vol 6, 211-221, 2008).
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joe Nocito
Sent: 14 May 2007 16:09
To: Rene J Buesa; Margiotta, Michele; histonet <@t> pathology.swmed.edu
Subject: Re: [Histonet] timer calibration
I'm sorry
am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough.
JTT
----- Original Message -----
From: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
To: "Margiotta, Michele" <MMargiotta <@t> bmhmc.org>; <histonet <@t> pathology.swmed.edu>
Sent: Tuesday, May 08, 2007 3:39 PM
Subject: Re: [Histonet] timer calibration
> Try any local watch repair store. They have to calibrate their
> repaired watches so it is very likely they can help you.
> René J.
>
> "Margiotta, Michele" <MMargiotta <@t> bmhmc.org> wrote:
> Hi All,
>
> Does anyone have a procedure for calibrating timers? We just had an
> inspection and got cited because our timers were not calibrated. Any
> info would be appreciated!
>
> Michele
>
>
>
> This e-mail and any files transmitted with it are confidential and are
> intended solely for the use of the individual or entity to which they
> are addressed.
> This communication may contain material protected by the
> attorney-client privilege. If you are not the intended recipient or
> the person responsible for delivering the e-mail to the intended
> recipient, be advised that you have received this e-mail in error and
> that any use, dissemination, forwarding, printing, or copying of this
> e-mail is strictly prohibited. If you have received this e-mail in
> error, please immediately notify the sender via return e-mail or call
> Brookhaven Memorial Hospital Medical Center at (631) 654-7282.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Ahhh...imagining that irresistible "new car" smell?
> Check outnew cars at Yahoo! Autos.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 19
Date: Wed, 9 May 2007 10:34:31 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] protein block
To: "'Gayle Callis'" <gcallis <@t> montana.edu>, "'Till, Renee'"
<TillRenee <@t> uams.edu>, <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002701c79257$ec227640$6501a8c0 <@t> Patsy>
Content-Type: text/plain; charset="us-ascii"
Dako's protein block is serum free and from what I understand is just
casein, no bsa. I use serum free protein block from Open Biosystems, I also
use their Stable Dab, Antibody Diluent, hematoxylin CS, etc., they are a lot
cheaper from most and I like their products.
Patsy
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle Callis
Sent: Tuesday, May 08, 2007 12:49 PM
To: Till, Renee; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] protein block
Renee,
I doubt it, sometimes protein blocks are serum-free blocks, and may contain
BSA and/or casein. I would assume another company's block should do the
job as well. Try Lab Vision, Biocare, etc to see what they have available.
At 12:36 PM 5/8/2007, you wrote:
>I have a protocol from a paper that I need to try and reproduce. They used
>the Protein Block from Dako, along with the LSAB+ kit. The Block seems to
no
>longer be available. What would be an alternative? Is this the same as the
>serum block I would normally use?
>
>
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
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------------------------------
Message: 20
Date: Wed, 9 May 2007 11:40:13 -0500
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: RE: [Histonet] timer calibration
To: "Edwards, R.E." <ree3 <@t> leicester.ac.uk>, "Joe Nocito"
<jnocito <@t> satx.rr.com>, "Rene J Buesa" <rjbuesa <@t> yahoo.com>, "Margiotta,
Michele" <MMargiotta <@t> bmhmc.org>, <histonet <@t> pathology.swmed.edu>
Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5C6 <@t> IS-E2K3.grhs.net>
Content-Type: text/plain; charset="iso-8859-1"
If we are going to ask if our timers are calibrated in the Histology Lab, then one could ask the question: "Do you calibrate the timers on your various automated instruments such as processors, stainers, cryostats, and the list could go on? I don't feel that 1 second a year is going to make a difference in my calibrated-timed tissue being processed or my H&E stains. If time does matter that precisely to you, then you most likely have a system in place for calibrating your timers.
Mike
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Edwards, R.E.
Sent: Wednesday, May 09, 2007 10:59 AM
To: Joe Nocito; Rene J Buesa; Margiotta, Michele; histonet <@t> pathology.swmed.edu
Subject: RE: [Histonet] timer calibration
Calibration of timers is essential, as one of the least publicised consequences of global warming is that time is speeding up by about a second a year( Wells et al;Journal of Man and Time Vol 6, 211-221, 2008).
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joe Nocito
Sent: 14 May 2007 16:09
To: Rene J Buesa; Margiotta, Michele; histonet <@t> pathology.swmed.edu
Subject: Re: [Histonet] timer calibration
I'm sorry
am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse. Both stains worked. I guess I'm just not anal enough.
JTT
----- Original Message -----
From: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
To: "Margiotta, Michele" <MMargiotta <@t> bmhmc.org>; <histonet <@t> pathology.swmed.edu>
Sent: Tuesday, May 08, 2007 3:39 PM
Subject: Re: [Histonet] timer calibration
> Try any local watch repair store. They have to calibrate their
> repaired watches so it is very likely they can help you.
> René J.
>
> "Margiotta, Michele" <MMargiotta <@t> bmhmc.org> wrote:
> Hi All,
>
> Does anyone have a procedure for calibrating timers? We just had an
> inspection and got cited because our timers were not calibrated. Any
> info would be appreciated!
>
> Michele
>
>
>
> This e-mail and any files transmitted with it are confidential and are
> intended solely for the use of the individual or entity to which they
> are addressed.
> This communication may contain material protected by the
> attorney-client privilege. If you are not the intended recipient or
> the person responsible for delivering the e-mail to the intended
> recipient, be advised that you have received this e-mail in error and
> that any use, dissemination, forwarding, printing, or copying of this
> e-mail is strictly prohibited. If you have received this e-mail in
> error, please immediately notify the sender via return e-mail or call
> Brookhaven Memorial Hospital Medical Center at (631) 654-7282.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Ahhh...imagining that irresistible "new car" smell?
> Check outnew cars at Yahoo! Autos.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
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_______________________________________________
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------------------------------
Message: 21
Date: Wed, 9 May 2007 11:41:06 -0500
From: "Kennedy, Lisa" <LisaKennedy <@t> catholichealth.net>
Subject: [Histonet] (no subject)
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAECF0E4DB60F046B042FDF36E574EBB524794 <@t> cmearmsx03.kearney-ne.catholichealth.net>
Content-Type: text/plain; charset=iso-8859-1
I am doing a search for information regarding Histologists who perform grossing in and dictation of surgical specimens. We have, in the past couple of years, begun to do that at our facility and are trying to get an idea of the increase of pay for histologist that doing this job warrants. Can anyone out there give me some pay ranges, etc. for Histologists who perform these tasks. Thanks so much.
Lisa Kennedy, HT(ASCP)
Good Samaritan Hospital
Kearney, NE 68847
------------------------------
Message: 22
Date: Wed, 9 May 2007 12:43:11 -0400
From: "Bryan Hewlett" <bhewlett <@t> cogeco.ca>
Subject: Re: [Histonet] timer calibration
To: "Joe Nocito" <jnocito <@t> satx.rr.com>, "Rene J Buesa"
<rjbuesa <@t> yahoo.com>, "Margiotta, Michele" <MMargiotta <@t> bmhmc.org>,
<histonet <@t> pathology.swmed.edu>
Message-ID: <002d01c79259$22348380$6500a8c0 <@t> mainbox>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=response
Joe,
You are talking fundamental philosophical differences here.
The inspectors are obviously Newtonian realists, while you're leaning more
to the Leibnitz/Kant view.
They are going to win!
Bryan
----- Original Message -----
From: "Joe Nocito" <jnocito <@t> satx.rr.com>
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>; "Margiotta, Michele"
<MMargiotta <@t> bmhmc.org>; <histonet <@t> pathology.swmed.edu>
Sent: Monday, May 14, 2007 11:08 AM
Subject: Re: [Histonet] timer calibration
I'm sorry
am I the only one that thinks calibrating timers is stupid. I mean how many
histology procedures are so time sensitive that the timers have to be
calibrated? Let's face it. I have had techs sit there and watch the clock
and rinse as soon as the timer goes off. And I've had techs wait for the
timer to go off , then mosey over and rinse. Both stains worked. I guess
I'm just not anal enough.
JTT
----- Original Message -----
From: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
To: "Margiotta, Michele" <MMargiotta <@t> bmhmc.org>;
<histonet <@t> pathology.swmed.edu>
Sent: Tuesday, May 08, 2007 3:39 PM
Subject: Re: [Histonet] timer calibration
> Try any local watch repair store. They have to calibrate their repaired
> watches so it is very likely they can help you.
> René J.
>
> "Margiotta, Michele" <MMargiotta <@t> bmhmc.org> wrote:
> Hi All,
>
> Does anyone have a procedure for calibrating timers? We just had an
> inspection and got cited because our timers were not calibrated. Any info
> would be appreciated!
>
> Michele
>
>
>
> This e-mail and any files transmitted with it are confidential and are
> intended
> solely for the use of the individual or entity to which they are
> addressed.
> This communication may contain material protected by the attorney-client
> privilege. If you are not the intended recipient or the person responsible
> for
> delivering the e-mail to the intended recipient, be advised that you have
> received
> this e-mail in error and that any use, dissemination, forwarding,
> printing,
> or copying of this e-mail is strictly prohibited. If you have received
> this e-mail in
> error, please immediately notify the sender via return e-mail or call
> Brookhaven Memorial Hospital Medical Center at (631) 654-7282.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Ahhh...imagining that irresistible "new car" smell?
> Check outnew cars at Yahoo! Autos.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
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------------------------------
Message: 23
Date: Wed, 9 May 2007 11:50:35 -0500
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: RE: [Histonet] (no subject)
To: "Kennedy, Lisa" <LisaKennedy <@t> catholichealth.net>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5C7 <@t> IS-E2K3.grhs.net>
Content-Type: text/plain; charset="us-ascii"
This is going to depend on the degree of grossing the tech does. If
they only put GI type bx's in or if they do the entire gross-in of
specimens to the degree a PA or Resident does. You may get a wide range
of pay.
Mike
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kennedy,
Lisa
Sent: Wednesday, May 09, 2007 11:41 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
I am doing a search for information regarding Histologists who perform
grossing in and dictation of surgical specimens. We have, in the past
couple of years, begun to do that at our facility and are trying to get
an idea of the increase of pay for histologist that doing this job
warrants. Can anyone out there give me some pay ranges, etc. for
Histologists who perform these tasks. Thanks so much. Lisa Kennedy,
HT(ASCP) Good Samaritan Hospital Kearney, NE 68847
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------------------------------
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