[Histonet] Interesting discussion about RE: timer calibration

[Bryan Hewlett]

So Joe isn't the only follower of Leibnitz/Kant,
I suspected as much!

Gayle, a mere second a year is nothing to us folks, I can't see those fine 
divisions on the timer anyway.
You might want to try counting instead, one thousand and one .. one thousand 
and two etc.
I've been doing that to time stains for years, the only downside is that 
colleagues who overhear, think you are losing it!

Bryan

----- Original Message ----- 
From: "Pamela Marcum" <pmarcum <@t> vet.upenn.edu>
To: "Gayle Callis" <gcallis <@t> montana.edu>; "Ingles Claire" 
<CIngles <@t> uwhealth.org>; <Histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, May 09, 2007 4:06 PM
Subject: Re: [Histonet] Interesting discussion about RE: timer calibration


> Shame on you Gayle!!  Now they will attempt to calibrate eyes and we are 
> in trouble 'cause they will have to decide with or without glasses and/or 
> contacts, not to mention the colour charts to be sure we know which shade 
> of blue or purple or red is correct.  Oh this is frightening!!
>
> Let's not tell ATF about recycling either.
>
> Gaining that second year to get older does scare me a little.
>
> Pam
>
> At 03:56 PM 5/9/2007, Gayle Callis wrote:
>>I have followed this discussion with interest and a bit of humor on the 
>>rationale for this chore.  It escapes me a bit -  but I did like the idea 
>>of an atomic clock but then saw the reply about battery failure.  Also, 
>>Greenwich time seemed the most logical over-all, always available and 
>>correct.  I was most bothered by a second added onto every year, and that 
>>means I get older by the second annually.  Hmmmm - but then the need for 
>>calibrated timers for validation purposes with complex testing?  Is it the 
>>automated stainers/processors they are worried about?
>>
>>As for resetting timers to do staining, my eyes are the timer when 
>>developing a chromogen during manual IHC procedures - this is  controlled 
>>with a microscope.  I have let a clock run up in time to garner a 
>>"ballpark" figure for optimal development, and found that can vary from 
>>day to day too, not an exact timing to be sure.
>>
>>I agree with Claire on silver staining, but depend on microscopic 
>>examination to monitor silver (for finalizing GMS, Jones methenamine 
>>silver) ,and other proper removal of dyes  Luxol fast blue, decolorizing 
>>tissue Gram stain.
>>
>>Now for a question:  Do you think CAP will ever require eye calibration? 
>>Sorry to even suggest that -
>>
>>Gayle Callis HTL, HT, MT(ASCP)
>>Research Histopathology Supervisor
>>Veterinary Molecular Biology
>>Montana State University
>>Bozeman MT 59717
>>
>>
>>    At 01:02 PM 5/9/2007, you wrote:
>>>
>>>Why don't we all just get atomic clocks and be done with it. I don't 
>>>believe they ever need to be calibrated. (unless the laws of physics and 
>>>radioactive element half-lives suddenly change).  I agree with Joe. 
>>>Staining is a special talent anyway. I have had to reset timers to add 
>>>more incubation time on stains lots of times (especially silver). Oh 
>>>sorry, it's only Wednesday. Only two more days to go.
>>>
>>>Claire Ingles
>>>  UW Hospital
>>>Madison WI
>>>
>>>________________________________
>>>
>>>From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Perry, 
>>>Margaret
>>>Sent: Wed 5/9/2007 12:42 PM
>>>To: histonet <@t> lists.utsouthwestern.edu
>>>Subject: [Histonet] timer calibration
>>>
>>>
>>>
>>>We calibrate our timers by doing the following.  Use the telephone to
>>>call 303-499-7111.  A voice will prompt you and tell you the time.  At
>>>the minute turn on the timer and record the Coordinated Universal time
>>>and the timer time.  Listen until the next minute and turn off the timer
>>>and record the Coordinated Universal Time and the time on the timer.
>>>
>>>
>>>
>>>
>>>
>>>Margaret Perry HT (ASCP)
>>>
>>>IHC Lab Manager Veterinary Science
>>>
>>>Animal Disease Research and Diagnostic Lab
>>>
>>>South Dakota State University
>>>
>>>Box 2175 North Campus Drive
>>>
>>>Brookings SD 57007
>>>
>>>
>>>
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>>>
>>>
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>>
>>
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>
> Best Regards,
>
> Pamela A Marcum
> Manager, Histology Special Procedures
> University of Pennsylvania
> School of Veterinary Medicine
> R.S. Reynolds Jr.  CORL
> New Bolton Center
> 382 West Street Road
> Kennett Square, PA 19348
>
> Phone - 610-925-6278
> Fax     - 610-925-8120
> E-mail - pmarcum <@t> vet.upenn.edu
>
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