[Histonet] protein block

[Patsy Ruegg]

Dako's protein block is serum free and from what I understand is just
casein, no bsa.  I use serum free protein block from Open Biosystems, I also
use their Stable Dab, Antibody Diluent, hematoxylin CS, etc., they are a lot
cheaper from most and I like their products.  
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle Callis
Sent: Tuesday, May 08, 2007 12:49 PM
To: Till, Renee; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] protein block

Renee,

I doubt it, sometimes protein blocks are serum-free blocks, and may contain 
BSA and/or casein.  I would assume another company's block should do the 
job as well.  Try Lab Vision, Biocare, etc to see what they have available.

At 12:36 PM 5/8/2007, you wrote:
>I have a protocol from a paper that I need to try and reproduce. They used
>the Protein Block from Dako, along with the LSAB+ kit. The Block seems to
no
>longer be available. What would be an alternative? Is this the same as the
>serum block I would normally use?
>
>

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610




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