[Histonet] Alizarin Red staining for mineralization nodules

[docqian]

Dear all,=0A=0AI would like to perform Alizarin Red staining for mineraliza=
tion nodules in primary osteoblasts, Does anyone have a protocol about it?=
=0AIn addition, for mineralization nodule formation, we should add ascorbic=
 acid and beta-glycerolphosphate into the culture medium, my question is : =
 use which solution to dissovle these drugs (distilled water, or culture me=
dium).=0A=0AThank you.=0A =0AGuofeng=0A=0A=0A      ________________________=
___________________________________ =0A=C7=C0=D7=A2=D1=C5=BB=A2=C3=E2=B7=D1=
=D3=CA=CF=E43.5G=C8=DD=C1=BF=A3=AC20M=B8=BD=BC=FE=A3=A1 =0Ahttp://cn.mail.y=
ahoo.com