[Histonet] RE: Regulatory Tcells (FoxP3) IHC

[C.M. van der Loos]

   Melissa,

   We  used  the  Abcam mouse antibody, clone 236A/E7 we great success on
   both  human  cryo  and  FFPE samples, and also in double staining with
   CD25,  CD4 (cryo).  For  more  details  please  see our paper recently
   accepted for JHC: Onno J. de Boer et al., Immunohistochemical Analysis
   of  Regulatory T Cell Markers FOXP3 and GITR on CD4^+CD25^+ T Cells in
   Normal  Skin  and  Inflammatory  Dermatoses.  Go  to  the  JHC website
   ([1]www.jhc.org) and go to exPRESS. You will find our paper under: May
   3rd 2007.

   Cheers,

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands

   Date: Mon, 7 May 2007 10:35:52 -0700
   From: "Melissa Gonzalez" <Melissa.Gonzalez <@t> cellgenesys.com>
   Subject: [Histonet] Regulatory Tcells (FoxP3) IHC
   To: <histonet <@t> lists.utsouthwestern.edu>
   Message-ID:
   <B9371E7140550D418212A1055BCD3E9E0C6EF4 <@t> hqsvr01mail.cgi.com>
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   Hi all,
   Anyone have any experience with this? How well does the Abcam antibody
   work on FFPE human tissues? What's a good positive control?
   Thanks
   Melissa

References

   1. http://www.jhc.org/