[Histonet] IHC antibodies for animals

[Patsy Ruegg]

Jan,
I used the Lab Vision polyclonal CD20.
Patsy

-----Original Message-----
From: Jan Shivers [mailto:shive003 <@t> umn.edu] 
Sent: Monday, May 07, 2007 3:01 PM
To: Patsy Ruegg
Subject: Re: [Histonet] IHC antibodies for animals

Hi Patsy,

Could you tell me which CD20 from Lab Vision you use?  I see they have one 
polyclonal and three monoclonal CD20s.  I'm in a search for another B cell 
marker, since Dako discontinued selling B Lymphocyte Antigen 36.

Thanks so much for any help you can provide.
Jan Shivers
U of MN Vet Diag Lab
shive003 <@t> umn.edu


----- Original Message ----- 
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
To: "'Patricia Adams'" <alaskagirl1950 <@t> yahoo.com>; "'HistoNet'" 
<histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, May 07, 2007 3:16 PM
Subject: RE: [Histonet] IHC antibodies for animals


> Patricia,
> I have used CD20 from Lab Vision on dogs, this does not require any
> pretreatment AR.  Also used cd79a from Dako for B cells on dog tissue. 
> For
> T cells I highly recommend Lab Vision's rab monoclonal CD3, it works great
> with citrate HIER on dogs.
> Patsy
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patricia
> Adams
> Sent: Monday, May 07, 2007 1:45 PM
> To: HistoNet
> Subject: [Histonet] IHC antibodies for animals
>
> Hello all!
> Now that I am working with animals, I am looking
> for antibodies that react on all species. But
> right now canine is the most important.
> I used a CD20 and a CD43, human tonsil as the
> control.  Of course my controls were beautiful,
> but the canine pt. did not work and it should
> have.
> CD20 and CD43 were all the choices I had to pick
> for B-cell and T-cell.
> Could you please give me some advice?  We have a
> very small budget and I do not have the choice of
> buying antibodies just to see if they will work
> on animals.
> Thank you very much,
>
> Patricia Adams
> -----
> Fight back spam! Download the Blue Frog.
> http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D
>
> __________________________________________________
> Do You Yahoo!?
> Tired of spam?  Yahoo! Mail has the best spam protection around
> http://mail.yahoo.com
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>