[Histonet] IHC antibodies for animals

Jan Shivers shive003 <@t> umn.edu
Mon May 7 15:56:49 CDT 2007


Patricia,

Are you looking for only CD markers that work on non-human mammalian 
species, or any antibodies in general?

My CD sources:
Rabbit polyclonal CD3 (LabVision/NeoMarkers; RB-9039-P; works well on 
everything.)
Mouse monoclonal CD79a (LabVision/NeoMarkers; MS-357-P; works moderately 
well, but some nonspecific staining)
Mouse monoclonal CD18 canine (Leukocyte Antigen Biology Lab/UCDavis; 
CA16.3C10; works well on canines only)
Mouse monoclonal CD18 feline (Leukocyte Antigen Biology Lab/UCDavis; 
FE3.9F2; works well on felines only)
Mouse monoclonal CD45RA canine (Leukocyte Antigen Biology Lab/UCDavis; 
CA21.4B3; works well on canines only)

Leukocyte Antigen Biology Lab also carries other CD markers, but they can 
only be used on frozen tissues; same goes for VMRD/Pullman, WA.

If you want information on tissue/tumor marker or immunoglobulin reactivity 
in non-human tissues, you can write me directly for the info.  I use about 
75 of them in my lab.

Jan Shivers
IHC/Histo/EM Section Head
Univ. of Minnesota Veterinary Diagnostic Lab
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu



----- Original Message ----- 
From: "Patricia Adams" <alaskagirl1950 <@t> yahoo.com>
To: "HistoNet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, May 07, 2007 2:44 PM
Subject: [Histonet] IHC antibodies for animals


> Hello all!
> Now that I am working with animals, I am looking
> for antibodies that react on all species. But
> right now canine is the most important.
> I used a CD20 and a CD43, human tonsil as the
> control.  Of course my controls were beautiful,
> but the canine pt. did not work and it should
> have.
> CD20 and CD43 were all the choices I had to pick
> for B-cell and T-cell.
> Could you please give me some advice?  We have a
> very small budget and I do not have the choice of
> buying antibodies just to see if they will work
> on animals.
> Thank you very much,
>
> Patricia Adams
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