[Histonet] IHC antibodies for animals

[Patsy Ruegg]

Patricia,
I have used CD20 from Lab Vision on dogs, this does not require any
pretreatment AR.  Also used cd79a from Dako for B cells on dog tissue.  For
T cells I highly recommend Lab Vision's rab monoclonal CD3, it works great
with citrate HIER on dogs.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patricia
Adams
Sent: Monday, May 07, 2007 1:45 PM
To: HistoNet
Subject: [Histonet] IHC antibodies for animals

Hello all!
Now that I am working with animals, I am looking
for antibodies that react on all species. But
right now canine is the most important.
I used a CD20 and a CD43, human tonsil as the
control.  Of course my controls were beautiful,
but the canine pt. did not work and it should
have.
CD20 and CD43 were all the choices I had to pick
for B-cell and T-cell.
Could you please give me some advice?  We have a
very small budget and I do not have the choice of
buying antibodies just to see if they will work
on animals.
Thank you very much,

Patricia Adams
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