[Histonet] reprocessing
Gayle Callis
gcallis <@t> montana.edu
Fri May 4 11:03:11 CDT 2007
Go to Histonet Archives and look for Mickie Johnson's way of reprocessing
tissues. I think he also published this in Histo Logic, Sakura Finetek
website - it was a delightfully simple thing to do and you did not have to
remove the paraffin to do it. Hopefully, he is looking in and will help
with this again.
At 09:35 AM 5/4/2007, you wrote:
>I am reprocessing some tissues that did not process adequately and was
>curious as to if after they are followed backwards through xylene, 100,
>95, 80 and 70, could the tissues go back into formalin with no adverse
>affects? Would it be better to simply leave them in 70% for the weekend.
>The one thing I don't want to do on a Friday afternoon is to sit around
>and stay late to process tissues.
>
>Thanks for your help.
>
>--
>Derek Papalegis
>Histotechnician
>T-NEMC Animal Histology Core
>Division of Laboratory Animal Medicine
>Tufts University 136 Harrison Avenue
>Boston, MA 02111
>phone: 617 636-2971
>fax: 617 636-8354
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
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