Pablo Sánchez Quinteiro
psanquin <@t> lugo.usc.es
Wed Mar 28 07:09:30 CDT 2007
I am doing OMP immunohistochemistry in paraffin-embedded sections of sheep
In my positive control -mouse olfactory tissue- there is not any
background, but in he sheep sections I get lot of unspecific labelling
(vessels, connective tissue, respiratory mucosae).
Being my primary antibody raised in goat I guess that there is an undesired
cross reaction with the sheep tissue.
Could I improve my blocking? I do half an hour in 2% BSA and 5% Horse
Should I use Goat normal serum?
Thanks in advance for your input.
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