[Histonet] Neuron specific beta III tubulin antibody
Nic Leipzig
nic.leipzig <@t> utoronto.ca
Tue Mar 27 09:46:37 CDT 2007
We are looking for an antibody for IHC on paraformaldehyde fixed cells
(differentiated rat neurospheres) that will stain only neurons. We have had
recent troubles with glial cells staining positive with beta III tubulin
antibodies.
Up until ~5 months ago we had an antibody to beta III tubulin (TUJ1) from
Chemicon that worked beautifully for differentiated Rat (Sprague Dawley)
neurospheres. Then the lots changed and it doesn't work at all and they
can't do anything about it besides sending us more vials of the same lot.
So we have tried a beta III tubulin TUJ1 antibody from Stem Cell
Technologies, but this stains neurons and glial cells (mostly astrocytes).
Sigma's SDL clone has even more background staining.
Any suggestions are greatly appreciated.
Thanks,
Nic
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Nic Leipzig, PhD
Postdoctoral Research Fellow
University of Toronto
Donnelly Centre for Cellular and Biomolecular Research
Shoichet Lab, 530-160 College Street
Toronto, ON M5S 3E1
Telephone: (416) 978-0352
Voicemail: (416) 978-6924 x224
E-mail: nic.leipzig <@t> utoronto.ca
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