[Histonet] Neuron specific beta III tubulin antibody

[Nic Leipzig]

We are looking for an antibody for IHC on paraformaldehyde fixed cells
(differentiated rat neurospheres) that will stain only neurons.  We have had
recent troubles with glial cells staining positive with beta III tubulin
antibodies.

 

Up until ~5 months ago we had an antibody to beta III tubulin (TUJ1) from
Chemicon that worked beautifully for differentiated Rat (Sprague Dawley)
neurospheres.  Then the lots changed and it doesn't work at all and they
can't do anything about it besides sending us more vials of the same lot.
So we have tried a beta III tubulin TUJ1 antibody  from Stem Cell
Technologies, but this stains neurons and glial cells (mostly astrocytes).
Sigma's SDL clone has even more background staining.

 

Any suggestions are greatly appreciated.

 

Thanks,

Nic

 

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Nic Leipzig, PhD

Postdoctoral Research Fellow

University of Toronto

Donnelly Centre for Cellular and Biomolecular Research

Shoichet Lab, 530-160 College Street

Toronto, ON M5S 3E1

 

Telephone: (416) 978-0352

Voicemail: (416) 978-6924 x224

E-mail: nic.leipzig <@t> utoronto.ca

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