[Histonet] Hollande's solution

Kellar, Eric C Eric.C.Kellar <@t> questdiagnostics.com
Mon Mar 26 07:37:45 CDT 2007

This is a modification of Bouin's solution. It is stable and will decalcify small bone specimens. Tissue that is fixed with Hollande's can be stained successfully with most stains, and the cupric acetate in the solution stabilizes red blood cell membranes and eosinophil and endocrine cell granules so that less lysis occurs than with Bouin's solution.
Thorough washing of the fixative prior to placing the specimen in a phosphate buffered formalin solution is necessary because the salts present in the solution will form an insoluble phosphate precipitate.

Eric C. Kellar
Quest Diagnostics
Histology Laboratory Supervisor
South Florida

 -----Original Message-----
From: 	histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]  On Behalf Of Bell, Lynne
Sent:	Monday, March 26, 2007 8:14 AM
To:	histonet <@t> lists.utsouthwestern.edu
Subject:	[Histonet] Hollande's solution

We are thinking of changing from Bouin's solution to Hollande's solution
for our GI biopsies.  I have looked at the MSDS and do not see any real
issues.  My biggest question is disposal of this solution.  Can it
safely go down the drain?  Also, Freida Carson's book suggests washing
the specimens before processing them.  Does everyone do that?
Thanks for any and all help.

Lynne A. Bell, HT (ASCP)
Central Vermont Medical Center
P. O. Box 547
130 Fisher Road
Barre, VT  05641

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