[Histonet] H&E artifact
Rene J Buesa
rjbuesa <@t> yahoo.com
Sat Mar 24 15:30:14 CDT 2007
The "empty nuclei" are very likely due to the sections NOT drained before they are dryed in the oven.
Place the slides vertical (against the water bath for instance) for a few minutes. Then gently shake them to eliminate any water that may remain in the slide (and underneath the section) BEFORE taking the slides to the drying oven.
If you go to HistoNet archieves you will find previous discussions on this "drying artifact".
joseph pastore <jpastor1 <@t> nycap.rr.com> wrote:
Hi Guys and Girls,
I really need some help here.We have been experiencing some artifact in our H&E stains. What it is are patches where the nuclei are very pale ble...that is the nuclei are just empty and a very pale blue and show no chromatin. Now tis is not throughout the section....only in spots. It also seems restricted to the biopsies. The routine stuff is fine. I mean in a batch of slides with both regular surgical specimens and biopsies, the artifact would be in only some of the biopsies not the routine stuff and not all the biopsies. Now at first we thought it was the stain. I checked that and whent through several variations but still the same. Then we looked at the processor. My Excelsior got rolly skrewed up in a recent power outage
So we gutted it and cleaned it out. During this time We used our old Leica processor. This worked like a charm.
Until last Fri. The pathologist noted that 3 of the biopsies showed the artifact. OK I was on leave but my tech said that the pathologist said the rest were fine. This week everything was fine until Fri. Again the routine specimens were OK but I saw this artifact in the biopsies.
I really need some input here
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