[Histonet] last tray etc.

JOHN P COLEMAN JPCOLEMA <@t> sentara.com
Fri Mar 23 14:58:42 CDT 2007


First of all, insomnia is an HT requirement and personality trait.
Second here goes nothing:

7 hospital system, 2 processing/cutting hubs. 18,000/annum cases north
hub, 62,000/annum cases south hub, average 280 blocks per 100 cases,
800/annum bone marrow cases 2.5 blocks per case.  

100% microwave processing, 2 Sakura units at each hub. 
South hub-1st run up to 60 cases grossed 8a-9a, cut 10a-12A, 100%
grossing by 3 registered PA's from 8Am to 4:30 PM, slides on 180 -220
blocks to one hospital cut 10 p to 3A delivered by 7:00 AM, biopsy
blocks, 30-40 cases sent to second site by 7 am, 250-500 main blocks
daily to South hub building docs by 10 am, IHC's ordered by 10 AM out by
4 PM avg 400 /day serving all hospitals from 1 central location, North
hub- 100% of 250 block load ready and delivered onsite and to second
north site by 7:30 AM.
Staffing south hub- 3 night HTs, 4 AM hts, 3 evening HTs 3 IHC HT's
Staffing North hub- 2 day  HT's, 1 ft night HT, 1 pt nite HT.
As stated by somebody else, majority is cut by evening/nights,dayshift
handles same day processing plus specials recuts, overflow, etc.
>>> <histonet-request <@t> lists.utsouthwestern.edu> 3/23/2007 2:05 PM >>>
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Today's Topics:

   1. RE: Looking to relocate  (Ingles Claire)
   2. RE: delivery time of last tray of H&E (Cheri Miller)
   3. Von Kossa on tissue culture (Nancy W. Troiano)
   4. AW: [Histonet] delivery time of last tray of H&E (Gudrun Lang)
   5. Question about historical photographs (Happel, James F.)
   6. RE: delivery time of last tray of H&E
      (Marshall Terry Dr,	Consultant Histopathologist)
   7. RE: Problem with intestinal epithelium (Patsy Ruegg)
   8. RE: Problem with intestinal epithelium (Patsy Ruegg)
   9. RE: Problem with intestinal epithelium
      (Yaskovich, Ruth A (NIH/NIDCR) [E])
  10. AW: [Histonet] Question about historical photographs (Gudrun
Lang)
  11. RE: Question about historical photographs (McCormick, James)


----------------------------------------------------------------------

Message: 1
Date: Fri, 23 Mar 2007 08:37:44 -0500
From: "Ingles Claire" <CIngles <@t> uwhealth.org>
Subject: RE: [Histonet] Looking to relocate 
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<08A0A863637F1349BBFD83A96B27A50A120011 <@t> uwhis-xchng3.uwhis.hosp.wisc.edu>
	
Content-Type: text/plain;	charset="iso-8859-1"

I'm looking to relocate to the Bahamas... Oh sorry, it's Friday and I
worked a 12 yesterday. :)  At least most of the snow is gone and it's
going to be 60 today! Oh yea, I have to work in a windowless lab...
 
Claire Ingles
UW Hospital and Clinics
Madison WI

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Dan
Malzahn
Sent: Thu 3/22/2007 10:22 PM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] Looking to relocate to Youngstown, Oh area



Hello All,
  
  Just wondering if anyone knows of any jobs that might be in the
Youngstown, OH area.  I am looking to relocate to that area and I am
having a hard time finding any Histology jobs in that area.  Any help
would be greatly appreciated.
  
  Thankyou





------------------------------

Message: 2
Date: Fri, 23 Mar 2007 08:43:41 -0500
From: "Cheri Miller" <cmiller <@t> physlab.com>
Subject: RE: [Histonet] delivery time of last tray of H&E
To: "'Marshall Terry Dr,	Consultant Histopathologist'"
	<Terry.Marshall <@t> rothgen.nhs.uk>, 	"'Akemi Allison-Tacha'"
	<akemiat3377 <@t> yahoo.com>,	"'Rene J Buesa'"
<rjbuesa <@t> yahoo.com>,
	"'Galiotto, Laura'" <LGaliotto <@t> nch.org>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002601c76d51$46cd0b90$db01a8c0 <@t> plab.local>
Content-Type: text/plain;	charset="iso-8859-1"

Turn around time Terry. For us it's about getting the slides to the
Pathologist early so they may order whatever additional testing needed
and
still get a hard copy report to the Clinician by the end of the day. We
have
24 hour turnaround for most cases. Cheri

Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Marshall
Terry Dr,Consultant Histopathologist
Sent: Thursday, March 22, 2007 12:28 PM
To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura;
histonet <@t> lists.utsouthwestern.edu 
Subject: RE: [Histonet] delivery time of last tray of H&E

This is all bloody unbelievable.  Don't any of you sleep?
What is the point of working at night?

Terry 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Akemi
Allison-Tacha
Sent: 22 March 2007 17:25
To: Rene J Buesa; Galiotto, Laura; histonet <@t> lists.utsouthwestern.edu 
Subject: Re: [Histonet] delivery time of last tray of H&E

RenΘ--That was similar to the protocol I followed in the old days
before
24/6 .  We provided rush's 1st, followed by bx's by 8AM, we did
however,
provide special stains and recuts and IHC's by 2-3 PM, depending if it
wasn't a lengthy procedure.  

If you have off site facilities to provide for, that is a different
situation.  You have to work around courier sheduals for delivering to
off
site facilities.  Everyone needs their slides and time is money!!

With the new microwave processing technology that is now in use, there
has
been a shift to having the majority of staff starting at 10 PM at
night.
MUCH TO THE DISLIKING OF HISTOTECH"S. 

The day shift is minimum.  IHC is done during the day shift, after the
pathologist has determined to do so. 
In most situations, a battery of IHC's tests are pre-set, as well as
with
special stains.

Akemi Allison-Tacha BS, HT (ASCP) HTL
President
Phoenix Lab Consulting & Staffing
Specializing in Histology, SS, IHC, & Microarray Madison, WI
Tele: 	(925) 788-0900
E-Mail:	akemiat3377 <@t> yahoo.com 

--- Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> Laura:
>   For a laboratory I used to manage with quite similar annual
workload 
> we had the following
> schedule:
>   1- last tissue accepted at 7:00 PM;
>   2- two VIPs atarting with delay to be finished at 4:00 AM next
day;
>   3- Rushes ready for the pathologists at 8:00 AM next day;
>   4-All slides ready, the latest, at 1:00 PM.
>   5- All IHC ready by 2 PM
>   6- all HC ready by noon.
>   RenΘ J.
> 
> "Galiotto, Laura" <LGaliotto <@t> nch.org> wrote:
>   Hello Fellow Histo Tech's,
> Will anyone be willing to share the delivery time of the last tray of

> H&E for the days workload to the pathologist reading them?
> What is your cutoff time for specimen acceptance?
> What is your annual case workload?
> I am trying to improve the TAT from specimen gross to the H&E 
> delivered to the pathologist. Unless we change the cutoff time I can

> not see how I can do this? Any suggestions?
> We process 22,000 cases a year an average of 280 blks a day
consisting 
> of types of tissue.
> Please Help
> Laura
> 
> 
> ******************* PLEASE NOTE ******************* This 
> E-Mail/telefax message and any documents accompanying this 
> transmission may contain information that is privileged,
confidential, 
> and/or exempt from disclosure under applicable law and is intended 
> solely for the addressee(s) named above. If you are not the intended

> addressee/recipient, you are hereby notified that any use of, 
> disclosure, copying, distribution, or reliance on the contents of
this 
> E-Mail/telefax information is strictly prohibited and may result in 
> legal action against you. Please reply to the sender advising of the

> error in transmission and immediately delete/destroy the message and

> any accompanying documents. Thank you.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
> 
> 
> 
> 
>  
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------------------------------

Message: 3
Date: Fri, 23 Mar 2007 08:51:56 -0500
From: "Nancy W. Troiano" <nancy.troiano <@t> yale.edu>
Subject: [Histonet] Von Kossa on tissue culture
To: histonet <@t> lists.utsouthwestern.edu 
Message-ID: <5.2.1.1.2.20070323085122.02819fd0 <@t> email.med.yale.edu>
Content-Type: text/plain; format=flowed; charset=us-ascii

Hi - we do VK on tissue cultures routinely - send me your fax number
and I 
will fax our protocol to you.




------------------------------

Message: 4
Date: Fri, 23 Mar 2007 16:43:54 +0100
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] delivery time of last tray of H&E
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000301c76d62$19503d20$6412a8c0 <@t> dielangs.at>
Content-Type: text/plain;	charset="iso-8859-1"

And then ... the patient has to get an appointment for the talk about
the
report with his/her doctor or has to get a date for the necessary
surgery.
In most cases there is no reason for such short TATs. 
Why all this hurry? The specimen aren't properly fixed, people have to
work
in nightshifts; There is no tumor, that grows in such a speed ...

My point of view.

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Ursprⁿngliche Nachricht-----

Von: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von
Cheri
Miller
Gesendet: Freitag, 23. MΣrz 2007 14:44
An: 'Marshall Terry Dr,Consultant Histopathologist'; 'Akemi
Allison-Tacha';
'Rene J Buesa'; 'Galiotto, Laura'; histonet <@t> lists.utsouthwestern.edu 
Betreff: RE: [Histonet] delivery time of last tray of H&E

Turn around time Terry. For us it's about getting the slides to the
Pathologist early so they may order whatever additional testing needed
and
still get a hard copy report to the Clinician by the end of the day. We
have
24 hour turnaround for most cases. Cheri

Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Marshall
Terry Dr,Consultant Histopathologist
Sent: Thursday, March 22, 2007 12:28 PM
To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura;
histonet <@t> lists.utsouthwestern.edu 
Subject: RE: [Histonet] delivery time of last tray of H&E

This is all bloody unbelievable.  Don't any of you sleep?
What is the point of working at night?

Terry 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Akemi
Allison-Tacha
Sent: 22 March 2007 17:25
To: Rene J Buesa; Galiotto, Laura; histonet <@t> lists.utsouthwestern.edu 
Subject: Re: [Histonet] delivery time of last tray of H&E

RenΘ--That was similar to the protocol I followed in the old days
before
24/6 .  We provided rush's 1st, followed by bx's by 8AM, we did
however,
provide special stains and recuts and IHC's by 2-3 PM, depending if it
wasn't a lengthy procedure.  

If you have off site facilities to provide for, that is a different
situation.  You have to work around courier sheduals for delivering to
off
site facilities.  Everyone needs their slides and time is money!!

With the new microwave processing technology that is now in use, there
has
been a shift to having the majority of staff starting at 10 PM at
night.
MUCH TO THE DISLIKING OF HISTOTECH"S. 

The day shift is minimum.  IHC is done during the day shift, after the
pathologist has determined to do so. 
In most situations, a battery of IHC's tests are pre-set, as well as
with
special stains.

Akemi Allison-Tacha BS, HT (ASCP) HTL
President
Phoenix Lab Consulting & Staffing
Specializing in Histology, SS, IHC, & Microarray Madison, WI
Tele: 	(925) 788-0900
E-Mail:	akemiat3377 <@t> yahoo.com 

--- Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> Laura:
>   For a laboratory I used to manage with quite similar annual
workload 
> we had the following
> schedule:
>   1- last tissue accepted at 7:00 PM;
>   2- two VIPs atarting with delay to be finished at 4:00 AM next
day;
>   3- Rushes ready for the pathologists at 8:00 AM next day;
>   4-All slides ready, the latest, at 1:00 PM.
>   5- All IHC ready by 2 PM
>   6- all HC ready by noon.
>   RenΘ J.
> 
> "Galiotto, Laura" <LGaliotto <@t> nch.org> wrote:
>   Hello Fellow Histo Tech's,
> Will anyone be willing to share the delivery time of the last tray of

> H&E for the days workload to the pathologist reading them?
> What is your cutoff time for specimen acceptance?
> What is your annual case workload?
> I am trying to improve the TAT from specimen gross to the H&E 
> delivered to the pathologist. Unless we change the cutoff time I can

> not see how I can do this? Any suggestions?
> We process 22,000 cases a year an average of 280 blks a day
consisting 
> of types of tissue.
> Please Help
> Laura
> 
> 
> ******************* PLEASE NOTE ******************* This 
> E-Mail/telefax message and any documents accompanying this 
> transmission may contain information that is privileged,
confidential, 
> and/or exempt from disclosure under applicable law and is intended 
> solely for the addressee(s) named above. If you are not the intended

> addressee/recipient, you are hereby notified that any use of, 
> disclosure, copying, distribution, or reliance on the contents of
this 
> E-Mail/telefax information is strictly prohibited and may result in 
> legal action against you. Please reply to the sender advising of the

> error in transmission and immediately delete/destroy the message and

> any accompanying documents. Thank you.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
> 
> 
> 
> 
>  
> ---------------------------------
> Sucker-punch spam with award-winning protection.
>  Try the free Yahoo! Mail Beta.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
> 


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




------------------------------

Message: 5
Date: Fri, 23 Mar 2007 11:49:23 -0400
From: "Happel, James F." <JHAPPEL <@t> PARTNERS.ORG>
Subject: [Histonet] Question about historical photographs
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<D5779DE61D63FC459A04D54F94FB11348109B1 <@t> PHSXMB27.partners.org>
Content-Type: text/plain;	charset="US-ASCII"

Good Day,

I am putting together a presentation on the past, present and future
of
histology/pathology and am looking for historical pictures, drawings,
advertisements, etc. to include in the presentation.  Does anyone have
images I
could use or know where I can find some?  Thank you.

James Happel
Massachusetts General Hospital





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------------------------------

Message: 6
Date: Fri, 23 Mar 2007 15:53:54 -0000
From: "Marshall Terry Dr,	Consultant Histopathologist"
	<Terry.Marshall <@t> rothgen.nhs.uk>
Subject: RE: [Histonet] delivery time of last tray of H&E
To: <gu.lang <@t> gmx.at>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<407F05A128805F4C879A33DBA32E618E20C2B0 <@t> TRFT-EX01.xRothGen.nhs.uk>
Content-Type: text/plain;	charset="iso-8859-1"

Absolutely.
This comes about when the lab is looking after the interests of the
Doctors, not the patients.

Terry 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Gudrun Lang
Sent: 23 March 2007 15:44
To: histonet <@t> lists.utsouthwestern.edu 
Subject: AW: [Histonet] delivery time of last tray of H&E

And then ... the patient has to get an appointment for the talk about
the report with his/her doctor or has to get a date for the necessary
surgery.
In most cases there is no reason for such short TATs. 
Why all this hurry? The specimen aren't properly fixed, people have to
work in nightshifts; There is no tumor, that grows in such a speed ...

My point of view.

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Ursprⁿngliche Nachricht-----

Von: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Cheri
Miller
Gesendet: Freitag, 23. MΣrz 2007 14:44
An: 'Marshall Terry Dr,Consultant Histopathologist'; 'Akemi
Allison-Tacha'; 'Rene J Buesa'; 'Galiotto, Laura';
histonet <@t> lists.utsouthwestern.edu 
Betreff: RE: [Histonet] delivery time of last tray of H&E

Turn around time Terry. For us it's about getting the slides to the
Pathologist early so they may order whatever additional testing needed
and still get a hard copy report to the Clinician by the end of the day.
We have
24 hour turnaround for most cases. Cheri

Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Marshall Terry Dr,Consultant Histopathologist
Sent: Thursday, March 22, 2007 12:28 PM
To: Akemi Allison-Tacha; Rene J Buesa; Galiotto, Laura;
histonet <@t> lists.utsouthwestern.edu 
Subject: RE: [Histonet] delivery time of last tray of H&E

This is all bloody unbelievable.  Don't any of you sleep?
What is the point of working at night?

Terry 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Akemi
Allison-Tacha
Sent: 22 March 2007 17:25
To: Rene J Buesa; Galiotto, Laura; histonet <@t> lists.utsouthwestern.edu 
Subject: Re: [Histonet] delivery time of last tray of H&E

RenΘ--That was similar to the protocol I followed in the old days
before
24/6 .  We provided rush's 1st, followed by bx's by 8AM, we did
however, provide special stains and recuts and IHC's by 2-3 PM,
depending if it wasn't a lengthy procedure.  

If you have off site facilities to provide for, that is a different
situation.  You have to work around courier sheduals for delivering to
off site facilities.  Everyone needs their slides and time is money!!

With the new microwave processing technology that is now in use, there
has been a shift to having the majority of staff starting at 10 PM at
night.
MUCH TO THE DISLIKING OF HISTOTECH"S. 

The day shift is minimum.  IHC is done during the day shift, after the
pathologist has determined to do so. 
In most situations, a battery of IHC's tests are pre-set, as well as
with special stains.

Akemi Allison-Tacha BS, HT (ASCP) HTL
President
Phoenix Lab Consulting & Staffing
Specializing in Histology, SS, IHC, & Microarray Madison, WI
Tele: 	(925) 788-0900
E-Mail:	akemiat3377 <@t> yahoo.com 

--- Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> Laura:
>   For a laboratory I used to manage with quite similar annual
workload 
> we had the following
> schedule:
>   1- last tissue accepted at 7:00 PM;
>   2- two VIPs atarting with delay to be finished at 4:00 AM next
day;
>   3- Rushes ready for the pathologists at 8:00 AM next day;
>   4-All slides ready, the latest, at 1:00 PM.
>   5- All IHC ready by 2 PM
>   6- all HC ready by noon.
>   RenΘ J.
> 
> "Galiotto, Laura" <LGaliotto <@t> nch.org> wrote:
>   Hello Fellow Histo Tech's,
> Will anyone be willing to share the delivery time of the last tray of

> H&E for the days workload to the pathologist reading them?
> What is your cutoff time for specimen acceptance?
> What is your annual case workload?
> I am trying to improve the TAT from specimen gross to the H&E 
> delivered to the pathologist. Unless we change the cutoff time I can

> not see how I can do this? Any suggestions?
> We process 22,000 cases a year an average of 280 blks a day
consisting 
> of types of tissue.
> Please Help
> Laura
> 
> 
> ******************* PLEASE NOTE ******************* This 
> E-Mail/telefax message and any documents accompanying this 
> transmission may contain information that is privileged,
confidential, 
> and/or exempt from disclosure under applicable law and is intended 
> solely for the addressee(s) named above. If you are not the intended

> addressee/recipient, you are hereby notified that any use of, 
> disclosure, copying, distribution, or reliance on the contents of
this 
> E-Mail/telefax information is strictly prohibited and may result in 
> legal action against you. Please reply to the sender advising of the

> error in transmission and immediately delete/destroy the message and

> any accompanying documents. Thank you.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
> 
> 
> 
> 
>  
> ---------------------------------
> Sucker-punch spam with award-winning protection.
>  Try the free Yahoo! Mail Beta.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
> 


_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
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------------------------------

Message: 7
Date: Fri, 23 Mar 2007 10:38:18 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] Problem with intestinal epithelium
To: "'Histonet'" <histonet <@t> pathology.swmed.edu>
Message-ID: <003401c76d69$aa75a9a0$6501a8c0 <@t> Patsy>
Content-Type: text/plain;	charset="iso-8859-1"

I would skip the pbs wash and get them into fixative asap and fix for
a
minium of 24hrs.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Jennifer
MacDonald
Sent: Wednesday, March 21, 2007 10:10 AM
To: Mikael Niku
Cc: histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu 
Subject: Re: [Histonet] Problem with intestinal epithelium

We get bovine intestine from a local university that has an agriculture

program.  We provide them with a large container of formalin and ask
that 
they open the intestine and place it immediately in the formalin.  We
have 
had great preservation of the epithelium.  The piece of intestine is
about 
8-10 inches long.
Jennifer


Jennifer MacDonald
Director, Histotechnician Training Program
Mt. San Antonio College
1100 N. Grand Ave.
Walnut, CA 91789
(909) 594-5611 ext. 4884
jmacdonald <@t> mtsac.edu 



Mikael Niku <mikael.niku <@t> helsinki.fi> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu 
03/21/2007 03:49 AM

To
histonet <@t> lists.utsouthwestern.edu 
cc

Subject
[Histonet] Problem with intestinal epithelium






Dear Histonetters,

we are trying to make good-quality sections (paraffin and cryo) of 
bovine intestines.
The problem is that the epithelium seems to be stripped off whatever we

do. This seems to be a problem especially with slaughterhouse material,

although the delay from death to sampling is less than 30 min.

Currently we are cutting the intestine to pieces, washing with PBS 
buffer, and fixing immediately in buffered paraformaldehyde or freezing

in isopentane / liquid nitrogen.

Any ideas, anyone? Or are we just too late here, could the mucosa be 
destroyed so soon after death?

With best regards,
Mikael Niku

-- 
////////////////////////////////////////////////////////////

  Mikael Niku             URL: www.helsinki.fi/~mniku/ 
  University of Helsinki  Dept. Basic Veterinary Sciences

  - MitΣk÷ mieltΣ olen lΣnsimaisesta sivistyksestΣ?
  Minusta se olisi erinomainen ajatus!  
                                          - Gandhi

////////////////////////////////////////////////////////////


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




------------------------------

Message: 8
Date: Fri, 23 Mar 2007 10:45:34 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] Problem with intestinal epithelium
To: "'Patsy Ruegg'" <pruegg <@t> ihctech.net>,	"'Histonet'"
	<histonet <@t> pathology.swmed.edu>
Message-ID: <003c01c76d6a$adacc350$6501a8c0 <@t> Patsy>
Content-Type: text/plain;	charset="iso-8859-1"

Oh yea, and certainly open the intestine up so the epithelium mucosa
is
readily exposed to the fixative.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy
Ruegg
Sent: Friday, March 23, 2007 10:38 AM
To: 'Histonet'
Subject: RE: [Histonet] Problem with intestinal epithelium

I would skip the pbs wash and get them into fixative asap and fix for
a
minium of 24hrs.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Jennifer
MacDonald
Sent: Wednesday, March 21, 2007 10:10 AM
To: Mikael Niku
Cc: histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu 
Subject: Re: [Histonet] Problem with intestinal epithelium

We get bovine intestine from a local university that has an agriculture

program.  We provide them with a large container of formalin and ask
that 
they open the intestine and place it immediately in the formalin.  We
have 
had great preservation of the epithelium.  The piece of intestine is
about 
8-10 inches long.
Jennifer


Jennifer MacDonald
Director, Histotechnician Training Program
Mt. San Antonio College
1100 N. Grand Ave.
Walnut, CA 91789
(909) 594-5611 ext. 4884
jmacdonald <@t> mtsac.edu 



Mikael Niku <mikael.niku <@t> helsinki.fi> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu 
03/21/2007 03:49 AM

To
histonet <@t> lists.utsouthwestern.edu 
cc

Subject
[Histonet] Problem with intestinal epithelium






Dear Histonetters,

we are trying to make good-quality sections (paraffin and cryo) of 
bovine intestines.
The problem is that the epithelium seems to be stripped off whatever we

do. This seems to be a problem especially with slaughterhouse material,

although the delay from death to sampling is less than 30 min.

Currently we are cutting the intestine to pieces, washing with PBS 
buffer, and fixing immediately in buffered paraformaldehyde or freezing

in isopentane / liquid nitrogen.

Any ideas, anyone? Or are we just too late here, could the mucosa be 
destroyed so soon after death?

With best regards,
Mikael Niku

-- 
////////////////////////////////////////////////////////////

  Mikael Niku             URL: www.helsinki.fi/~mniku/ 
  University of Helsinki  Dept. Basic Veterinary Sciences

  - MitΣk÷ mieltΣ olen lΣnsimaisesta sivistyksestΣ?
  Minusta se olisi erinomainen ajatus!  
                                          - Gandhi

////////////////////////////////////////////////////////////


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




------------------------------

Message: 9
Date: Fri, 23 Mar 2007 12:57:15 -0400
From: "Yaskovich, Ruth A \(NIH/NIDCR\) [E]"
	<ryaskovich <@t> dir.nidcr.nih.gov>
Subject: RE: [Histonet] Problem with intestinal epithelium
To: "Patsy Ruegg" <pruegg <@t> ihctech.net>,	"Histonet"
	<histonet <@t> pathology.swmed.edu>
Message-ID:
	<CDECB5092C07B74CBF59AD7C7F821976032605E8 <@t> NIHCESMLBX3.nih.gov>
Content-Type: text/plain;	charset="iso-8859-1"

Make sure you have plenty of volume and change fix after 1st day to
fresh. May-be give them a second day of fix. (sometimes tissues are
bloody and dirty)
Ruth Yaskovich
National Institutes of Health
National Institute of Dental and Craniofacial Research
Neurobiology and Pain Therapeutics Branch

-----Original Message-----
From: Patsy Ruegg [mailto:pruegg <@t> ihctech.net] 
Sent: Friday, March 23, 2007 12:46 PM
To: 'Patsy Ruegg'; 'Histonet'
Subject: RE: [Histonet] Problem with intestinal epithelium

Oh yea, and certainly open the intestine up so the epithelium mucosa
is
readily exposed to the fixative.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy
Ruegg
Sent: Friday, March 23, 2007 10:38 AM
To: 'Histonet'
Subject: RE: [Histonet] Problem with intestinal epithelium

I would skip the pbs wash and get them into fixative asap and fix for
a
minium of 24hrs.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Jennifer
MacDonald
Sent: Wednesday, March 21, 2007 10:10 AM
To: Mikael Niku
Cc: histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu 
Subject: Re: [Histonet] Problem with intestinal epithelium

We get bovine intestine from a local university that has an agriculture

program.  We provide them with a large container of formalin and ask
that 
they open the intestine and place it immediately in the formalin.  We
have 
had great preservation of the epithelium.  The piece of intestine is
about 
8-10 inches long.
Jennifer


Jennifer MacDonald
Director, Histotechnician Training Program
Mt. San Antonio College
1100 N. Grand Ave.
Walnut, CA 91789
(909) 594-5611 ext. 4884
jmacdonald <@t> mtsac.edu 



Mikael Niku <mikael.niku <@t> helsinki.fi> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu 
03/21/2007 03:49 AM

To
histonet <@t> lists.utsouthwestern.edu 
cc

Subject
[Histonet] Problem with intestinal epithelium






Dear Histonetters,

we are trying to make good-quality sections (paraffin and cryo) of 
bovine intestines.
The problem is that the epithelium seems to be stripped off whatever we

do. This seems to be a problem especially with slaughterhouse material,

although the delay from death to sampling is less than 30 min.

Currently we are cutting the intestine to pieces, washing with PBS 
buffer, and fixing immediately in buffered paraformaldehyde or freezing

in isopentane / liquid nitrogen.

Any ideas, anyone? Or are we just too late here, could the mucosa be 
destroyed so soon after death?

With best regards,
Mikael Niku

-- 
////////////////////////////////////////////////////////////

  Mikael Niku             URL: www.helsinki.fi/~mniku/ 
  University of Helsinki  Dept. Basic Veterinary Sciences

  - MitΣk÷ mieltΣ olen lΣnsimaisesta sivistyksestΣ?
  Minusta se olisi erinomainen ajatus!  
                                          - Gandhi

////////////////////////////////////////////////////////////


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



------------------------------

Message: 10
Date: Fri, 23 Mar 2007 17:57:30 +0100
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] Question about historical photographs
To: "'Happel, James F.'" <JHAPPEL <@t> PARTNERS.ORG>
Cc: histonet <@t> lists.utsouthwestern.edu 
Message-ID: <000001c76d6c$79613de0$6412a8c0 <@t> dielangs.at>
Content-Type: text/plain;	charset="iso-8859-1"

http://www.unifi.it/unifi/dbag/oldtools/en/microtomes.html 
http://micro.magnet.fsu.edu/primer/museum/index.html 
http://caliban.mpiz-koeln.mpg.de/~stueber/frey/index.html 

perhaps something you can use
Gudrun Lang
 

-----Ursprⁿngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von
Happel,
James F.
Gesendet: Freitag, 23. MΣrz 2007 16:49
An: histonet <@t> lists.utsouthwestern.edu 
Betreff: [Histonet] Question about historical photographs

Good Day,

I am putting together a presentation on the past, present and future
of
histology/pathology and am looking for historical pictures, drawings,
advertisements, etc. to include in the presentation.  Does anyone have
images I
could use or know where I can find some?  Thank you.

James Happel
Massachusetts General Hospital





The information transmitted in this electronic communication is
intended
only for the person or entity to whom it is addressed and may contain
confidential and/or privileged material. Any review, retransmission,
dissemination or other use of or taking of any action in reliance upon
this
information by persons or entities other than the intended recipient
is
prohibited. If you received this information in error, please contact
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Compliance HelpLine at 800-856-1983 and properly dispose of this
information.


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Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




------------------------------

Message: 11
Date: Fri, 23 Mar 2007 12:26:46 -0500
From: "McCormick, James" <JMcCormick <@t> schosp.org>
Subject: RE: [Histonet] Question about historical photographs
To: <gu.lang <@t> gmx.at>,	"Happel, James F." <JHAPPEL <@t> PARTNERS.ORG>
Cc: histonet <@t> lists.utsouthwestern.edu 
Message-ID:
	<913FAC2B773C19488E26AE6572180FA50AE80428 <@t> exch01.schosp.org>
Content-Type: text/plain;  charset="utf-8"

Mr. Happel, 
We have an extensive library of books and illustrations that concern
the history of Histotechnology.....PAST  PRESENT  FUTURE....

I am an Author of many articles and teach workshops for the NSH on the
history of our craft.  Contact Mr. Drew Mehta for source images that
will be helpful.  These come from my Science Heritage Library
Collection.
email dnmehta <@t> Coretech-holdings.com  .  I have copied Drew this note. 

GOOD LUCK, 
J.B.McCormick,M.D. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Gudrun Lang
Sent: Friday, March 23, 2007 10:58 AM
To: 'Happel, James F.'
Cc: histonet <@t> lists.utsouthwestern.edu 
Subject: AW: [Histonet] Question about historical photographs

http://www.unifi.it/unifi/dbag/oldtools/en/microtomes.html 
http://micro.magnet.fsu.edu/primer/museum/index.html 
http://caliban.mpiz-koeln.mpg.de/~stueber/frey/index.html 

perhaps something you can use
Gudrun Lang
 

-----Urspr├╝ngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von
Happel,
James F.
Gesendet: Freitag, 23. März 2007 16:49
An: histonet <@t> lists.utsouthwestern.edu 
Betreff: [Histonet] Question about historical photographs

Good Day,

I am putting together a presentation on the past, present and future
of
histology/pathology and am looking for historical pictures, drawings,
advertisements, etc. to include in the presentation.  Does anyone have
images I
could use or know where I can find some?  Thank you.

James Happel
Massachusetts General Hospital





The information transmitted in this electronic communication is
intended
only for the person or entity to whom it is addressed and may contain
confidential and/or privileged material. Any review, retransmission,
dissemination or other use of or taking of any action in reliance upon
this
information by persons or entities other than the intended recipient
is
prohibited. If you received this information in error, please contact
the
Compliance HelpLine at 800-856-1983 and properly dispose of this
information.


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


_______________________________________________
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Thank you for your cooperation.




------------------------------

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End of Histonet Digest, Vol 40, Issue 35
****************************************

John P.J. Coleman HT(ASCP)QIHC
Clinical Specialist
Anatomic Pathology
Sentara Laboratory Services
Cell/Voicemail(757)287-6657
pager: (757)456-6695
office:-(757)388-3295



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