[Histonet] Problem with intestinal epithelium

[Yaskovich, Ruth A (NIH/NIDCR) [E]]

Make sure you have plenty of volume and change fix after 1st day to fresh. May-be give them a second day of fix. (sometimes tissues are bloody and dirty)
Ruth Yaskovich
National Institutes of Health
National Institute of Dental and Craniofacial Research
Neurobiology and Pain Therapeutics Branch

-----Original Message-----
From: Patsy Ruegg [mailto:pruegg <@t> ihctech.net] 
Sent: Friday, March 23, 2007 12:46 PM
To: 'Patsy Ruegg'; 'Histonet'
Subject: RE: [Histonet] Problem with intestinal epithelium

Oh yea, and certainly open the intestine up so the epithelium mucosa is
readily exposed to the fixative.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Friday, March 23, 2007 10:38 AM
To: 'Histonet'
Subject: RE: [Histonet] Problem with intestinal epithelium

I would skip the pbs wash and get them into fixative asap and fix for a
minium of 24hrs.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer
MacDonald
Sent: Wednesday, March 21, 2007 10:10 AM
To: Mikael Niku
Cc: histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Problem with intestinal epithelium

We get bovine intestine from a local university that has an agriculture 
program.  We provide them with a large container of formalin and ask that 
they open the intestine and place it immediately in the formalin.  We have 
had great preservation of the epithelium.  The piece of intestine is about 
8-10 inches long.
Jennifer


Jennifer MacDonald
Director, Histotechnician Training Program
Mt. San Antonio College
1100 N. Grand Ave.
Walnut, CA 91789
(909) 594-5611 ext. 4884
jmacdonald <@t> mtsac.edu



Mikael Niku <mikael.niku <@t> helsinki.fi> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
03/21/2007 03:49 AM

To
histonet <@t> lists.utsouthwestern.edu
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Subject
[Histonet] Problem with intestinal epithelium






Dear Histonetters,

we are trying to make good-quality sections (paraffin and cryo) of 
bovine intestines.
The problem is that the epithelium seems to be stripped off whatever we 
do. This seems to be a problem especially with slaughterhouse material, 
although the delay from death to sampling is less than 30 min.

Currently we are cutting the intestine to pieces, washing with PBS 
buffer, and fixing immediately in buffered paraformaldehyde or freezing 
in isopentane / liquid nitrogen.

Any ideas, anyone? Or are we just too late here, could the mucosa be 
destroyed so soon after death?

With best regards,
Mikael Niku

-- 
////////////////////////////////////////////////////////////

  Mikael Niku             URL: www.helsinki.fi/~mniku/
  University of Helsinki  Dept. Basic Veterinary Sciences

  - Mitäkö mieltä olen länsimaisesta sivistyksestä?
  Minusta se olisi erinomainen ajatus!  
                                          - Gandhi

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