[Histonet] RE:QDots

[Melissa Gonzalez]

Alejandro,
Look at the instructions carefully for mounting & coverslipping your slides when using Qdots. They are very sensitive to quenching if you don't use the proper mounting media. I kind of gave up on them, and would recommend you use Alexa Fluors instead. Or talk to Invitrogen's tech support regarding your specific applications, they have very good tech support...
GL,
Melissa

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Message: 27
Date: Thu, 15 Mar 2007 17:28:31 +0100
From: Alejandro Ortiz Stern <stern <@t> ipmc.cnrs.fr>
Subject: [Histonet] Qdots
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <45F9742F.6050905 <@t> ipmc.cnrs.fr>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

I would like to know if anyone has experience with Streptavidine-Qdots 
for the labeling of Acetone post-fixed slides (IHF). How do you wash the 
slides in order to get the best signal to noise ratio?

Thank you for your input on the matter.

-- 
Alejandro Ortiz Stern M.D. Ph.D.,
Postdoctoral Fellow
Institut National de la Santé et de la Recherche Médicale, E0344
Université de Nice-Sophia-Antipolis
Institut de Pharmacologie Moléculaire et Cellulaire
660 Route des Lucioles - 06560 Valbonne - FRANCE
TEL: 33 (4) 93 95 77 80 - FAX: 33 (4) 93 95 77 08