AW: [Histonet] processing times for needle biopsies -using sponges

Gudrun Lang gu.lang <@t>
Sun Mar 18 06:09:31 CDT 2007

We use sponges with nearly all our biopsies (40-60). We have no short-run in
the VIP, so they are processed with the standard procedure (13 hours in
Because the cassettes with the sponges like to swim up, we put them always
in the lowest row.
I have never experienced hard, brittle prostata biopsies.

Gudrun Lang
Biomed. Analytikerin
Akh Linz
Krankenhausstr. 9
4020 Linz
-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] Im Auftrag von Patricia
Gesendet: Sonntag, 18. März 2007 05:06
An: histonet <@t>; histonet <@t>
Betreff: [Histonet] processing times for needle biopsies -using sponges

 I would like to hear about VIP processing times other folks use for
prostate biopsies using biopsy sponges.
We currently run an average of 60 cassettes( some days can be 120) all with
2 sponges.- (would prefer to use mesh window cassettes but pathologists
prefer the flat and complete sections we (usually) get more easily with the
We rotate 1 of each reagent type ( 95%,100%, Xylene,wax) on processor after
each run - this does seem to help with carryover problem.
Run is 3hrs 45 min. long
Every once in a while we get  few nasty hard fragmented cores and other
times under dehydration.- These cannot all be blamed on collection or
grossing technique ( though we have tried).
Seems like processing is uneven.(PV is on)
Does anyone use longer times with so many sponges?
Has any one tried something to space out cassettes( maybe an empty cassette
in-between full ones) to get better contact with solutions?
Any other ideas??

Pat Valente
San Antonio TX

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