[Histonet] Eosinophils

[Gayle Callis]

We use an H&E (Richard Allan hematoxylin 1 and eosin Y) in order to count 
eosinophils in lungs.  Our sections are cut at 2 - 3 um to avoid so much 
cellular overlap,  then do a standard H&E stain with longer time in the 
eosin - 2 minutes instead of 1.  This time can be adjusted for your lab 
needs.  Nothing else has worked as well for us.  The biggest problem has 
been people not very well versed in eosinophil morphology in an H&E stained 
section.


    At 01:23 AM 3/15/2007, you wrote:
>Morning All
>Anyone have a stain for eosinophils in  FFP mouse lung tissue and also
>nerve stain in FFP mouse intestine for a technologist in our research
>lab. Thanks
>Marilyn
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)