[Histonet] RE: Glassware cleaning

Kellar, Eric C Eric.C.Kellar <@t> questdiagnostics.com
Tue Mar 13 14:49:38 CDT 2007

Hello Kaye,

Here's my documentation procedure...

Rinse all dishes with tap water thoroughly prior to washing.
Use bleach and or appropriate strength detergent for stubborn stains prior to washing.  Rinse thoroughly after acid cleaning.
Wash dishes, either manually or by dishwasher.
Rinse dishes thoroughly with DI or distilled water and dry dishes.
After washing and rinsing multiple pieces of glass/plastic ware, it is only necessary to randomly select one piece to test for residual detergent. Either one of the following two methods may be used.
My lab aides rotate weekly through glassware cleaning and have both options.

pH Meter Method
Rinse a small clean beaker by filling and emptying 3 times with DI or distilled water.
Fill the same beaker with DI or distilled water and measure the pH using a pH meter.  Record the pH as "Source Water" pH on "Residual Detergent Testing Log" date and initial.
Fill the piece of washed glass/plastic ware to be tested approximately 10% full with "source water" (i.e., 10ml into 100ml beaker).   Add more "source water" if needed to completely immerse the pH meter electrode. Swirl "source water" in glass/plastic ware to extract detergent residues from all possible surfaces.
Take pH reading with pH meter and record as "Glassware pH" on "Residual Detergent Testing Log" date and initial.
Re-wash and rinse tested piece of glass/plastic ware before using
Color Indicator Method
Place 2.5 ml of DI or distilled water in the glass/plastic ware to be tested and swirl to extract detergent residues from all possible surfaces.
Using a plastic pipette, add one drop of 0.5% Phenolphthalein indicator and look for a reddish color in the zone of first mixing.  Do not mix vigorously.
Record presence or absence of reddish color indicator on "Residual Detergent Testing Log" date and initial.
Re-wash and rinse tested piece of glass/plastic ware before using.

Eric C. Kellar
Quest Diagnostics 
Histology Laboratory Supervisor
South Florida

		-----Original Message-----
		From: 	histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]  On Behalf Of Kaye Ryan
		Sent:	Tuesday, March 13, 2007 8:45 AM
		To:	Histonet
		Subject:	[Histonet] Glassware cleaning

		Hi Everyone,

		According to the General CAP Checklist (GEN.41770) "documented procedures for handling and cleaning of glassware, including methods for testing for detergent removal" must be performed.  An example of a method for doing this is given.  Can you please tell me how you are documenting this for the histology lab?  We do have a procedure for doing this but I wanted to see how others are documenting this.

		Thanks in advance,
		Kaye Ryan

		Kaye Ryan
		Histology Manager/Educational Coordinator
		Shands Rocky Point Laboratories
		(352) 265-0111, 72093

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