Recommendation for Re: [Histonet] mouse on mouse kit

[Gayle Callis]

Helen,

A wonderful poster at the NSH symposium/convention in Ft Lauderdale (by 
Mary Payette, et al) showed a comparison of 8 kits.  The top two giving no 
background were DAKO's ARK kit and Biocares MM kit, both based on 
biotinylation of the primary antibody.

One had minimal background, two gave moderate background, and three had 
marked background.  This was a very well designed test of kits.  In our 
experience, kits containing secondary antibodies always gave more 
background, even with all the tidy little special blocks companies put in 
the kits.  We would be using the top two above in the future.

  07:08 AM 3/13/2007, you wrote:
>Hi Histonetters,
>      I need a recommendation on a IHC mouse on mouse detection kit.
>                                                     Helen Johnson

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)