[Histonet] Intercalar heart muscle discs

koellingr <@t> comcast.net koellingr <@t> comcast.net
Sun Mar 11 15:25:56 CDT 2007

I agree totally with Rene the Brilliant Black is beautiful.  I tried it years later on a couple of slides and it was outstanding.  It is just at the time of the project, azocarmine and thionin were right on the shelf and not a whole lot of labs have and use Brilliant Black and also I didn't want to fool with mercury fixatives since these future medical doctors (and pathologists) needed to be familiar with FFPE more than with mercury fixation.  It all boils down again to what is the purpose of the stain.

Ray Koelling
Phenopath Labs
Seattle, WA

-------------- Original message -------------- 
From: Rene J Buesa <rjbuesa <@t> yahoo.com> 

> Sharon: 
> The best reccommenden method is that of Dietrich tested by Schmorl in 1928 
> Sol.A: 1% brilliant black 3B in 0.1% acetic acid 
> Sol.B 1% aq. safranin 
> Sol.C :methanol. 
> 3-4 µm section from mercury chloride fixed material to solA x 1-5 min ¨ 
> rinse ¨ sol.B x 3-10 min ¨ drain ¨ absolute ethanol til 
> differentiated ¨ sol.C ¨xylene ¨ coverslip 
> (Peter Gray's 21.16). 
> A good Phophotungstic acid hematoxylin will also do. 
> RenEJ. 
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