[Histonet] Intercalar heart muscle discs

Rene J Buesa rjbuesa <@t> yahoo.com
Sun Mar 11 11:44:20 CDT 2007

  The best reccommenden method is that of Dietrich tested by Schmorl in 1928
  Sol.A: 1% brilliant black 3B in 0.1% acetic acid
  Sol.B 1% aq. safranin
  Sol.C :methanol.
  3-4 µm section from mercury chloride fixed material to solA x 1-5 min &#8594; rinse &#8594; sol.B x 3-10 min &#8594; drain &#8594; absolute ethanol til differentiated &#8594; sol.C &#8594;xylene &#8594; coverslip
  (Peter Gray's 21.16).
  A good Phophotungstic acid hematoxylin will also do.
  René J.

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