[Histonet] Intercalar heart muscle discs

[Rene J Buesa]

Sharon:
  The best reccommenden method is that of Dietrich tested by Schmorl in 1928
  Sol.A: 1% brilliant black 3B in 0.1% acetic acid
  Sol.B 1% aq. safranin
  Sol.C :methanol.
  3-4 µm section from mercury chloride fixed material to solA x 1-5 min → rinse → sol.B x 3-10 min → drain → absolute ethanol til differentiated → sol.C →xylene → coverslip
  (Peter Gray's 21.16).
  A good Phophotungstic acid hematoxylin will also do.
  René J.



 
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