[Histonet] bone fixation

[mesruh turkekul]

Hi Sharon,

You can collect the tissues you do not want to fix very quick if possible
and then perfuse.
If you only immerse fix the femur try to use large volumes of fixative and
fix for longer at least 48 hours at 4C by rotating or stirring. Do not use
narrow and conical bottom tubes. Use big and wide flat bottom containers.
You can add 0.5M EDTA to the fixative as well.

Regards,
Mesruh Turkekul

On 3/9/07, histonet-request <@t> lists.utsouthwestern.edu <
histonet-request <@t> lists.utsouthwestern.edu> wrote:
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>   1. does anyone know how to get better immersion fixation of
>      mouse femur? (scoop <@t> mail.nih.gov)
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> ----------------------------------------------------------------------
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> Message: 1
> Date: Fri, 9 Mar 2007 11:19:15 -0500
> From: scoop <@t> mail.nih.gov
> Subject: [Histonet] does anyone know how to get better immersion
>        fixation of mouse femur?
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <p06020408c21736fb8025@[156.40.160.97]>
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> Dear Histonetters,
>
> I have been using fixed mouse femur to do mouse bone marrows.  I get
> much better fixation and preservation of bone marrow if I perfuse the
> mouse than if I immersion fix the femurs.  However, I prefer not to
> perfusion fix the mouse because perfusion fixation prevents me from
> collecting other tissues unfixed.  Is there a way to improve the
> fixation of the mouse femurs when I immersion fix?  I'm guessing that
> the problem is I'm not getting good enough penetration - I suppose I
> could poke a few holes in the femurs?
>
> Thanks in advance for your help,
> Sharon
>
>
>
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> End of Histonet Digest, Vol 40, Issue 12
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