[Histonet] Mystery Fixative - Free vs Bound Formaldehyde

[Randy Khoo]

Hi All,

I was looking at past postings for something which has
been bothering me but there doesn't seemed to be any
archival material on the subject.

If I understand correctly, formaldehyde can exists in
free and bound form. Can the bound form be released
into the free form over a period of time? 

Has anyone ever used formaldehyde releasers to
preserve materials instead of formaldehyde as such?

Regards,
Randy

--- Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> Glen:
>   Perhaps she is referring to "Jores's fixative".
> There are 2 formulas, one from 1896 and the other
> from 1913
>   The original one (1896) is as follows:
>   Water -- 100 mL + 40% formaldehyde--10 mL + spdium
> sulfate -- 2g + magnesium sulfate -- 2g + sodium
> chloride -- 1 g
>   Hope this will help you!
>   René J.
> 
> "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
> wrote:
>   All,
> 
> Our AP supervisor is looking for an older fixative
> that she used to use to revitalize staining on
> tissue that had been sitting in formalin for months.
> 
> 
> She seems to think it is called "Yori's, Youri's or
> Jori's" fixative and they used to make it on-site
> back in the day but the recipe has long since been
> lost.
> 
> Any Help Would be Greatly Appreciated,
> 
> Glen Dawson
> IHC Manager
> Milwaukee, WI
> 
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