[Histonet] Chatter in GI Biopsies

[Tony Henwood]

A few thoughts,

Check the microtome - not loose?, is the knife angle right?
When the blocks are cooling prior to sectioning is the ice too cold?
(This occurred in my lab when we were freezing our ice trays in a -60oC
freezer)
Is the trimming (or facing) too vigorous? Polish the block before
sectioning (take a few sections at 4-5um at normal sectioning speed and
discard)


Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

>	Bandaged Bear Day - Friday 30th March 2007
>	For more information and to order merchandise visit
www.bandagedbearday.com.au
>
>


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
eileen.lonergan <@t> verizon.net
Sent: Friday, 9 March 2007 5:35 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Chatter in GI Biopsies


Dear All,

We have been noticing an increase in chatter for our GI biopsies over
the last month or so.  We use both microwave (Milestone) and
conventional (VIPs) processors.  We were using the microwave mainly for
small biopsies with very good results until the increase in chatter
occured.  We switched to the conventional processors and got about the
same results.  The temps look fine as well as the solutions.   Does
anyone out there have an idea on this problem.  At this point in time, I
am open to just about all suggestions. I can be emailed at my work:
mailto:elonergan <@t> convergedx.     Thanks.

Eileen Lonergan HT(ASCP)
ConVerge Diagnostic Services
Peabody MA 01960



Eileen Lonergan
(207) 749-9617  cell
(207) 324-6468  home

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

**********************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
**********************************************************************