[Histonet] IHC bone tissue adherence issues

[Alan Bright]

Dear Joe,

If you can locate Dr John Dodds, who is an expert at frozen sections of
bone & has been in the USA for the last 10 years I am sure he will be
able to assist you. He has been using a number of our cryostats at
various locations without the need of a tape transfer system on
undecalcified bone.

Hopefully someone seeing this posting may know his location and inform
you.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright <@t> brightinstruments.com
Web Site: www.brightinstruments.com
Skype User ID: dazzle0


-----Original Message-----
From: Kang, Joseph J [mailto:joseph.j.kang <@t> pfizer.com] 
Sent: 07 March 2007 19:09
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC bone tissue adherence issues


Dear Histoneters,
I have a great favor to ask.
I need to find out what procedures I can use to keep bone sections
intact on glass slides during IHC staining. There seems to be a lot of
tissue lifting or loss during antigen retrieval. I am currently using
plus slides on regular paraffin sections and leaving them in the oven
for up to 24 hours with no success. I also attempted using the
Instrumedics tape transfer system, which seems to retain more tissue
than regular sections, but with similar results. 
We also attempted doing IHC on frozen knee joints using the tape
transfer system to skip the antigen retrieval steps without success. Any
positive feedback on the matter is greatly appreciated.

Joe




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