[Histonet] fungus control for PASfungus

Tony Henwood AnthonyH <@t> chw.edu.au
Wed Mar 7 16:32:43 CST 2007

Acceptable but not optimal.

Fungus tends to stain differently to basement membranes with some
variability depending on the fungus type and its age (dead fungus tends
to stain poorly - need a Chromic acid - Methenamine Silver or a Chromic
acid - Schiffs reaction to better show them).

It is easy to make your own Fungi control. Fix and process some slices
of mouldy bread or fruit.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bansal,
Sent: Thursday, 8 March 2007 1:25 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] fungus control for PASfungus

Is it acceptable to use kidney tissue as a control for PAS stain when
looking for fungus? We have been having a debate on this issue and would
like to know what you all think. Meera Bansal MD Mercy Medical Center
Rockville Centre, NY

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