[Histonet] block-orientation

Gayle Callis gcallis <@t> montana.edu
Tue Mar 6 14:20:45 CST 2007

I did a compromise, angled the biopsy in the block so I could gently 
stretch it on the water bath.  We sectioned at 2 um, could do 1 um, and 
used a harder paraffin with either high profile or low profile blades, 
Accuedge from Sakura always gave us superb sections.

At 12:37 PM 3/6/2007, you wrote:
>A quite simple question:
>What is the better way of block-orientation in a rotation-microtome for
>kidney needle biopsies?
>With the biopsy parallel or normal to the knife? To get a good ribbon
>without wrinkels in the tissue?
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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