[Histonet] paraffin vs. frozen
katri <@t> cogeco.ca
Tue Mar 6 13:08:07 CST 2007
from clinical laboratory point of view, most of the time we don't have a
choice. Most of our work is done retrospectively on formalin fixed paraffin
embedded material (FFPE). This is why many manufacturers produce antibodies
meant for this purpose. In selection of the antibodies, the type of material
you have to work with has to be kept in mind.
But the fact is, that not all antigens can survive or they are masked by the
FFPE process. The masking has been largely overcome by many types of
retrieval procedures : enzymatic digestion and use of heat in different
buffers. But the antigens, which don't survive FFPE, must be demonstrated in
frozen sections, fixed in various fixatives (or not fixed at all)depending
on the antigen to be demonstrated. Usually these procedures are much faster,
but morphology often suffers.
In a clinical lab, a procedure has to be in place for collecting specimens
for frozen section work. For instance a technologist has to attend, when
renal or muscle biopsies are performed by a clinician and proper and prompt
handling of the specimen is essential.
I hope this is of some help to you. I'm sure other histonet members will
come forward for more information.
Hamilton, Ontario, Canada
----- Original Message -----
From: "Kalleberg, Kristopher" <Kristopher.Kalleberg <@t> unilever.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, March 06, 2007 1:22 PM
Subject: [Histonet] paraffin vs. frozen
If anyone could give some insight as to what the differences are between
frozen sections and paraffin sections when performing IHC I would
greatly appreciate it. I am running a study and the results for a few
of the markers I am not to happy with and am contemplating changing over
to frozen sections for upcoming studies. Is there any rhyme or reason
as to why antibodies work better with frozens sections or paraffin
sections? Any help will of course be greatly appreciated.
40 Merritt Blvd.
Trumbull, CT 06611
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