[Histonet] Collagen Staining

[Tony Henwood]

Try a Van Gieson's stain. To remove the yellow picric acid staining,
rinse the slides in water before DC&M.
Depending on choice of dye, you can get red, blue and even green
collagen staining.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jo-Ann
Bader, Ms.
Sent: Tuesday, 6 March 2007 1:02 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Collagen Staining


Good Morning Everyone,

I am looking for a stain for Collagen without a background stain.  I
have tried the Aniline blue from the Masson's Trichrome with
unsatisfactory results.  

Jo-Ann Bader


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