[Histonet] GAG Stain

Geoff McAuliffe mcauliff <@t> umdnj.edu
Mon Mar 5 12:13:22 CST 2007

Alcian Blue at pH 2.5 or 1.0, precceded by digestion with specific 
enzymes, will work for formalin-fixed (better is formalin-alcohol-acetic 
acid) tissues.
There is also the "critical electrolyte concentration" methods with 
Alcian Blue.
Some lectin methods (see Vector Labs website) will demo specific amino 
sugars of the GAG.


AGrobe2555 <@t> aol.com wrote:

>Hello All,
>I'm trying to find a staining protocol for Gycosaminoglycans (GAGs).   Does 
>anyone have a protocol that will work on fixed sheep tissues?
>Albert C.  Grobe, PhD
>International Heart Institute of Montana Foundation
>Tissue  Engineering Lab, Saint Patrick  Hospital
><BR><BR><BR>**************************************<BR> AOL now offers free 
>email to everyone.  Find out more about what's free from AOL at 
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu

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