[Histonet] GAG Stain
Geoff McAuliffe
mcauliff <@t> umdnj.edu
Mon Mar 5 12:13:22 CST 2007
Alcian Blue at pH 2.5 or 1.0, precceded by digestion with specific
enzymes, will work for formalin-fixed (better is formalin-alcohol-acetic
acid) tissues.
There is also the "critical electrolyte concentration" methods with
Alcian Blue.
Some lectin methods (see Vector Labs website) will demo specific amino
sugars of the GAG.
Geoff
AGrobe2555 <@t> aol.com wrote:
>Hello All,
>I'm trying to find a staining protocol for Gycosaminoglycans (GAGs). Does
>anyone have a protocol that will work on fixed sheep tissues?
>Thanks,
>Albert
>
>Albert C. Grobe, PhD
>International Heart Institute of Montana Foundation
>Tissue Engineering Lab, Saint Patrick Hospital
>
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu
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