[Histonet] Problems using Pap Pen
Rene J Buesa
rjbuesa <@t> yahoo.com
Sun Mar 4 08:16:14 CST 2007
Try using a simple wax pencil instead of a PAP pen. Absolutely innocuous!
Tilman Krieger <tilman.fuldatal <@t> web.de> wrote:
I tried to do some ICCs (DAB / Ni) but had some problems, obviously
caused by using a Pap pen (Manufactured by Beckman Coulter) in a wrong
way. All my tissues that were put on Superfrost-slides (Manufacturer:
Menzel) looked really strange: only half of the tissue was coloued. It
seemed as if something had faded into the tissue. I do not exactly know
what it was and at which point of the ICC it happent, but I suppose it
was the pap pen. Other tissues from the same organ (the same animal!)
were correctly coloured - they were fixated on different slides
Which distance do you keep between tissue and pap pen line? How long do
you let it dry?
Thanks in advance, Til MR
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
Never miss an email again!
Yahoo! Toolbar alerts you the instant new Mail arrives. Check it out.
More information about the Histonet