[Histonet] IHC screening on TMA slides

[Jackie M O'Connor]

It would make more sense to put 3-4 cores of multiple hybridomas on a tma 
slide resulting in 100 cores,  and stain each tma slide with various 
antibodies to characterize your hybridomas.   I do that for 100's of 
xenograft tumors.



"Patsy Ruegg" <pruegg <@t> ihctech.net> 
03/02/2007 12:41 PM

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<ana.merino-trigo <@t> wanadoo.fr>, "'Jackie M O'Connor'" 
<Jackie.O'Connor <@t> abbott.com>
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RE: [Histonet] IHC screening on TMA slides






Physically separating 100 cores on one slide sounds like a nightmare to 
me.
Even a very thin water barrier pen sounds tuff, but that is what I thought
of.  I would have thought you would have been better off making a tissue
block with a small core of tissue and drop the small sections into 96 well
elisa plates and is done for elisa/IHC.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ana
MERINO-TRIGO
Sent: Friday, March 02, 2007 11:28 AM
To: Jackie M O'Connor
Cc: histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC screening on TMA slides

Because we do want to select in a quick way the right hybridoma in between
more than 100 hybridomas using the same tissue. We thought in using the
advantages of TMA technology, but the other way around. Same tissue in
multiple spots to test different antibodies. So, we will need to find the
way to separate physically the spots to perform different immunos in the
same slide. Does this make sense? 
Thanks, 
Ana
----- Original Message ----- 
From: Jackie M O'Connor 
To: ana.merino-trigo <@t> wanadoo.fr 
Cc: histonet <@t> lists.utsouthwestern.edu ;
histonet-bounces <@t> lists.utsouthwestern.edu 
Sent: Friday, March 02, 2007 7:18 PM
Subject: Re: [Histonet] IHC screening on TMA slides



I don't understand why you would have a TMA with 100 cores of the same
sample. 




Ana MERINO-TRIGO <ana.merino-trigo <@t> wanadoo.fr> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu 
03/02/2007 11:57 AM Please respond to
ana.merino-trigo <@t> wanadoo.fr

To"histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
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Subject[Histonet] IHC screening on TMA slides







 
Hi Histonet, 

I was wondering if anyone could give me any ideas or suggestions in order 
to
perform IHC screening over a TMA slide containing about 100 spots of 0.6 
or
1.0 mm of the same tissue. The idea it will be to select hybridomes for
further characterization based on IHC reactivity on a given positive 
control
tissue.  Around 100 hybridomas to test on a TMA  slide containing  spots
from the same sample. I cannot see the way to do the immuno without spread
the antibody in between the spots. I heard that exits multispot type of 
lids
that you could put over the slide to perform the immno but I don't really
know where to look for these lids or if they are good enough to be sure 
that
antibody doesn't spread in between spots. 

Any information it will be really appreciate it, 

All the best, 
Ana
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