[Histonet] GRAM Stain Timing Question

Joe Nocito jnocito <@t> satx.rr.com
Thu Mar 1 18:43:07 CST 2007

we have a wash bottle with acetone and rinse the slides until the Crystal 
Violet stops dripping off the slides, just like Gram smears in Micro.
    I'm in a haze again. This time it's Scotch. Is it Friday yet?

----- Original Message ----- 
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, February 28, 2007 1:15 PM
Subject: [Histonet] GRAM Stain Timing Question

I seem to do a lot of GRAM stains and although I've modified some of the
times to suit my pathologists' tastes, I have never been able to pin
down the first differentiation time in 100% alcohol.  Today, I'm using
10 quick dips although it's only because the wind is blowing and the
moon is on the rise.  Can someone give me a more specific guideline for
differentiating after the Gram's Iodine and before the Safranin? I'd
like to leave a more definite legacy when I retire than "differentiate
in 100% alcohol or acetone"...  I'm thankin' you in advance.  And I
think we all know who the Purple Haze Generation are on THIS media,
don't we???

Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 700

Albuquerque, NM  87106


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