[Histonet] External diameter of large vein
Rene J Buesa
rjbuesa <@t> yahoo.com
Thu Mar 1 10:57:48 CST 2007
As you are surely aware the whole process (fixation, processing, embedding and sectioning) will have an effect on the real diameter as compared with the fresh specimen.
The fact that you will end with a diameter not exactly as the initial will give you some "latitude" in the measurement.
In some occasions when I had needed to measure whole sections y just used a copying machine and copy the section. Then I was able to measure the copied image.
This is a "low tech" approach but that have served me well in the past. I have been able to use a good ruler for the measurements.
Sorry, no references though. This is mine!
Tahseen <tahseen <@t> brain.net.pk> wrote:
How can the external diameter of large vein like renal vein be measured on
slide under light microscope?
As the vein is large and measures several millimeters the micrometer
scale cannot be used. we also do not want to measure grossly using a venire
calipre. Our aim is to measure the vertical, oblique and transverse external
diameter on the slide through microscope. Please also give the reference of
Dr Robina Shaheen
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