[Histonet] External diameter of large vein

[Rene J Buesa]

Tahseen:
  As you are surely aware the whole process (fixation, processing, embedding and sectioning) will have an effect on the real diameter as compared with the fresh specimen.
  The fact that you will end with a diameter not exactly as the initial will give you some "latitude" in the measurement.
  In some occasions when I had needed to measure whole sections y just used a copying machine and copy the section. Then I was able to measure the copied image.
  This is a "low tech" approach but that have served me well in the past. I have been able to use a good ruler for the measurements.
  Sorry, no references though. This is mine!
  René J. 

Tahseen <tahseen <@t> brain.net.pk> wrote:
  Dear All,
How can the external diameter of large vein like renal vein be measured on 
slide under light microscope?
As the vein is large and measures several millimeters the micrometer 
scale cannot be used. we also do not want to measure grossly using a venire 
calipre. Our aim is to measure the vertical, oblique and transverse external 
diameter on the slide through microscope. Please also give the reference of 
the method.

Thanks

Dr Robina Shaheen
Abbottabad,
Pakistan
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