[Histonet] X-gal staining

[Surita Banwait]

I would be very interested in some suggestions as well... we have tried
whole mount staining on mouse brain without much success.  We also tried
paraffin processing samples and then staining with beta gal antibody
from chemicon (also without much success).

Thanks!
Surita

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Surita Banwait
Morphology & Imaging Core
Research Associate II         
Buck Institute for Age Research
8001 Redwood Blvd.
Novato, CA 94945
415-209-2221
sbanwait <@t> buckinstitute.org
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
jhnspam <@t> aol.com
Sent: Thursday, March 01, 2007 8:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] X-gal staining

Does anyone have a good procedure for X-Gal staining of frozen tissue
sections? I keep getting only non specific staining with the procedure I
have.  I would also like a procedure for whole mount staining as well.
Thanks very much for your help!!!
 
Pam Johnson
Supervisor, ARC Histology
332 N. Lauderdale St.
Memphis, TN 38105-2794
901-495-2272, office
901-495-3112, fax
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