[Histonet] Nuclear artefact II

[Gale, Nadia]

I've looked at everyone's suggestions/comments and am sad to report that
I cannot get rid of the nuclear "bubble" artefact from our slides.
 
I have tried
 
- decreasing water bath temp
- increasing/decreasing drying oven time before H&E staining
- varying fixation times in formalin
 
I am currently trying
 
- decreasing drying oven temperature
- changing the "wrap" that I use for the biopsies when grossing
- looking at our wax times on the processor
 
I agree with Bob that it's extremely frustrating that our community
cannot seem to agree on a common cause for this (what I am now
realizing) common artefact.
 
I'll keep you posted with my results.
 
Thank you so much for all of your ideas and help with this, I am most
grateful.
 
 
Sincerely,
Nadia Gale
Histotechnologist
Centre for Translational and Applied Genomics (CTAG)
3427-600 West 10th Avenue
Vancouver, BC V5Z 4E6
tel 604-877-6000 ext 2426
fax 604-877-6089
ngale <@t> bccancer.bc.ca