[Histonet] Silver staining technique

[Joe Nocito]

I've been burned in the past (I'm not talking about being flamed now) by 
just checking the control slide without checking the patient. Like most 
other places, we cut controls in batches. Some one else might cut the 
patient. Different techs, different cutting techniques, etc. Me, myself, 
have performed GMS stains were I just looked at the control (which was 
perfect I might add) and the patient was over stained because it was loaded 
with fungi more than the control did.
    People say I'm a fun guy or was that fungi? Either way, I have a 
tendency to grow on people.

Joe
----- Original Message ----- 
From: <bamoe <@t> gundluth.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, June 27, 2007 3:13 PM
Subject: [Histonet] Silver staining technique


>
> Hi all -
>
> A question has come up in our laboratory regarding silver staining
> technique.
>
> We currently run special stains manually.  For the GMS silver stain,
> control tissue is run on a separate slide from the patient tissue. During
> the silver step  (we use a microwave for heating) the control slide is
> checked microscopically for adequate development.
>
> The question is this:
>
> If the control tissue is developed to satisfaction of the technician, do
> you automatically remove the patient slides from the silver and continue 
> on
> with the procedure,  OR do you individually check each patient slide,
> giving some more time in the silver solution if needed?
>
> Any and all comments are welcome!
>
> Thank you!
>
> Barb Moe
> Gundersen Lutheran Medical Center
> La Crosse WI
>
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