[Histonet] Re: Nuclear artefactI

[Sarah Jones]

Dear Samurai Pathologist and other interested folks:

Here is the answer to your question about nuclear bubbling artifact:

http://www.anatechltdusa.com/Innovators/2_Innartifact.html


Best regards,

Sarah A. Jones, HTL(ASCP)CM


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
rsrichmond <@t> aol.com
Sent: Wednesday, June 27, 2007 3:06 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Nuclear artefactI 


 
Nadia Gale, Histotechnologist at the Centre for Translational and
Applied Genomics (CTAG) in Vancouver, BC asks:

>>I am seeing nuclear artefact in some of our formalin fixed paraffin
embedded tissues. The artefact appears as vacuoles or bubbles in the
nuclei with H&E. I have tried multiple things to fix this to no avail.<<
 
I think we've discussed this topic before on Histonet, and maybe I just
wasn't paying attention - but I would really like to know how to trouble
shoot this common artifact: just what do you do first, and next, and
after that?

In my travels as a locum tenens pathologist this is one of the most
common problems I see in laboratories. Always everybody is in denial
that any such thing exists. In particular, this bubble artifact makes it
impossible to assess the nuclear grade of breast cancers and other
tumors requiring nuclear grading.

Bob Richmond
Samurai Pathologist
Knoxville TN



 


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