[Histonet] fixative for endothelial cell membrane for protein andlipid staining

[Patsy Ruegg]

I would think that ordinary NBF would to the job.  You might want to
consider something other than paraffin processing though to preserve the
lipids, perhaps formalin fixed, sucrose infiltrated frozen sections???
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Culberson,
Cathy
Sent: Monday, June 25, 2007 9:12 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] fixative for endothelial cell membrane for protein
andlipid staining

I need to fix primary liver endothelial cells to stain for cell membrane
lipids and also for a membrane protein, caveolin.  What fixative would
be best for this?  Thanks for your help, as always.  

Cathy Culberson, MS
University of North Carolina at  Charlotte
Dept of Biology
704-687-8675
fax 704-687-3128
email  crculber <@t> uncc.edu


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