[Histonet] frozen sections on monkey eye

[Jaimie Hoh]

Hello Jennifer,

I've worked in an eye institute before and i have done
a lot of frozen mouse and rat eyes. Normally i'll have
a lot of difficulties in cutting the lens as when it
is fixed in 4% PFA, it hardens.
I've read in one of the previous email from histonet
that Perenyi's fluid is a good fixative for eye as
both Nitric and chromic acids have a softening effect
on the tissuesand ethanol is a coagulant fixative. It
is good as it fixes retina really well with little
separation of the layers and does not harden the lens.
 
I hope this will help.

Jaimie

 
--- "Harvey, Jennifer Lynn"
<jennifer.harvey <@t> Vanderbilt.Edu> wrote:

> Histonet:
> 
>  
> 
> I thought years of bone work I had done some of the
> most challenging
> histology. We move to Nashville and I took a job in
> eye research. Was I
> crazy??? This is worse. I need to cut frozen
> sections on 4%PFA fixed
> monkey eyes?  Any ideas?? 
> 
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> 
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> 
> Thanks!!!!
> 
>  
> 
>  
> 
> Jennifer Harvey
> 
> Vanderbilt Eye Institute
> 
> RM 8105 MCE North Tower
> 
> 1215 21st Ave. South
> 
> Nashville, TN  37232-8808
> 
> Phone: 615-936-1486
> 
>  
> 
>  
> 
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>  
> 
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