[Histonet] microglia in mouse brains

[koellingr <@t> comcast.net]

Yes, you can use F4/80 on microglia in brain if you aware of potential problems regarding the activation state of the microglia. Also try BM8 and on most data sheets they will explain the much mis-understood difference between F4/80 and BM8.  Using those 2 antibodies can give you very similar but very subtle and observable differences in the stained population of cells whether in brain or spleen in tissue IHC or in flow.

Ray Koelling
Phenopath Labs
Seattle, WA 

-------------- Original message -------------- 
From: "Liz Chlipala" <liz <@t> premierlab.com> 

> Brett 
> 
> I'm not positive but I think that you might be able to use F4/80 as microglia 
> marker in mouse brain. 
> 
> Liz 
> 
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC 
> Manager 
> Premier Laboratory, LLC 
> P.O. Box 18592 
> Boulder, CO 80308 
> phone (303) 735-5001 
> fax (303) 735-3540 
> liz <@t> premierlab.com 
> www.premierlab.com 
> 
> Ship to Address: 
> 
> Premier Laboratory, LLC 
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> 
> -----Original Message----- 
> From: histonet-bounces <@t> lists.utsouthwestern.edu 
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Connolly, Brett 
> M 
> Sent: Wednesday, June 20, 2007 9:46 AM 
> To: histonet <@t> lists.utsouthwestern.edu; ihcrg <@t> googlegroups.com 
> Subject: [Histonet] microglia in mouse brains 
> 
> A colleague is looking for an antibody & protocol for staining microglia in 
> mice. 
> 
> Any suggestions? 
> 
> Thx, 
> Brett 
> 
> Brett M. Connolly, Ph.D. 
> Research Fellow 
> MRL, Imaging Research 
> Merck & Co., Inc. 
> WP-44K 
> PO Box 4 
> West Point, PA 19486 
> PH 215-652-2501 
> fax. 215-993-6803 
> e-mail. brett_connolly <@t> merck.com 
> 
> 
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