[Histonet] Processing frozen tissue to paraffin

[Monfils, Paul]

I have done this a number of times, with good results.  No artifact should be introduced by the thawing process, if the tissue is thawed in formalin.  Of course, if artifacts were introduced in the process of freezing, because of freezing too slow or other factors, those artifacts will remain.

> ----------
> From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Connolly, Brett M
> Sent: 	Wednesday, June 20, 2007 11:32 AM
> To: 	histonet <@t> lists.utsouthwestern.edu
> Subject: 	[Histonet] Processing frozen tissue to paraffin
> 
> Hi all,
> I have some fresh frozen tissue at -80C I would like to process to
> paraffin blocks and then run some subsequent IHC. 
> 
> Can I just plop it into 10%NBF and process as usual?
> 
> Thx,
> Brett
> 
> Brett M. Connolly, Ph.D.
> Research Fellow
> MRL, Imaging Research
> Merck & Co., Inc.
> WP-44K
> PO Box 4
> West Point, PA 19486
> PH 215-652-2501
> fax. 215-993-6803
> e-mail. brett_connolly <@t> merck.com
> 
> 
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