[Histonet] Negative control IHC

[Coskran, Timothy M]

What do you do when you have a polyclonal antibody for which the vendor
has not determined an Ig concentration?  It's easy to match
concentration and isotype for a monoclonal but can be impossible for a
polyclonal.  If we have an Ig concentration, we will match with Rabbit
IgG.  If not, do you count on a buffer control, irrelevant antibody (not
concentration matched), or normal sera?  Lots of choices, not sure what
is the best for this situation.

 

Tim Coskran

Pfizer

 

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