[Histonet] P53 survey results

[James L Burchette]

Five responses to the p53 survey. Here are the results:

1.
I use Dako's monoclonal p53 (clone DO-7) with Dako's pH 6.0 citrate target 
retrieval for my HIER.

2.
I use Dako's p53 clone DO-7 as well with Dako's Target Retrieval
Solutin in a steamer.

3.
We use Biocare's p53 at 1:50 with Biocare's Bull's Eye, 
Diva or BORG for 2" in their Decloaking chamber (pressure cooker). 
Or we use Ventana's standard CC1 HIER on our VMS BenchMark and XT.

4.
Clone from DO-7 from DAKO, diluted 1:80 with pH6 HIER

5.
Dako clone DO-7 ph 6 (biocare decloaker ie: pressure cooker)1:100 30/15
using envision plus detection system from Dako and stained on Dako
autostainer. Breast cancer as control.
Dako clone DO-7 ph 6 (biocare decloaker ie: pressure cooker)1:100 30/15
using envision plus detection system from Dako and stained on Dako
autostainer. Breast cancer as control.


JB