[Histonet] Brain Pigment

[Liz Chlipala]

Vernon

I'm assuming that your pigment is brown, if that's the case then I would
switch to an alkaline phosphatase detection system with a red chromagen such
as fast red.  If that's not then there are other colored chromagens that
could be used.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vernon
Dailey
Sent: Tuesday, June 12, 2007 10:29 AM
To: Histonet
Subject: [Histonet] Brain Pigment

Hi Histonet,

I am trying IHC on some human brain tissue. The region of interest is 
the substantia nigra. The pigment in the SN cells is interfering with my 
IHC. I have a few protocols for removing this pigment but they are very 
damaging to the sections. Does anyone have a protocol that works well 
for this purpose? Any help would be appreciated. Best,

Vernon

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